Localization of transcripts from the wingless gene in whole Drosophila embryos

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

In situ hybridization has been used to detect transcription in whole Drosophila embryos. Improved results have been obtained by incubating the hybridized embryos in liquid emulsion prior to autoradiographic exposure. This technique has been used to map the distribution of transcripts from the segment-polarity gene wingless (wg), which is transcribed in a stripe in each segment of the trunk region. By the extended germband stage additional areas of transcription in the head and caudal regions define a total of 21 domains, comprising the foregut, six regions in the head, three thoracic and ten abdominal segments, and the hindgut. At the end of the extended germband stage, the pattern of wg transcription is no longer uniform in the dorsoventral axis: wg transcripts are absent from the lateral epidermis. This pattern of wg transcription is discussed with particular regard to the segmentation of the terminalia. Also it is suggested that the dorsoventral reorganization could be related to the specification of the imaginal disc primordia.

Original languageEnglish (US)
Pages (from-to)289-298
Number of pages10
JournalDevelopment
Volume103
Issue number2
StatePublished - 1988
Externally publishedYes

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Drosophila
Embryonic Structures
Head
Terminalia
Imaginal Discs
Emulsions
Epidermis
Genes
In Situ Hybridization
Thorax

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Localization of transcripts from the wingless gene in whole Drosophila embryos. / Baker, Nicholas E.

In: Development, Vol. 103, No. 2, 1988, p. 289-298.

Research output: Contribution to journalArticle

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