The site of crossover between the δ- and β-globin gene sequence resulting in Lepore Boston globin gene formation has been localized at the DNA level. Probes specific for detecting the intervening sequences (IVS) of the δ- and β-globin genes were used to map the origin of cellular DNA fragments of a patient homozygous for hemoglobin Lepore Boston. Restriction endonuclease analysis and hybridization of this DNA to IVS specific probes show that most, if not all, of the large intervening sequences (IVS 2) in Lepore DNA are derived from the β-globin gene IVS 2. In addition, the crossover occurs in a region of DNA in which the δ- and β-genes have almost complete nucleotide homology, and might be expected to pair most tightly during meiosis.
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