Localization of the site of recombination in formation of the lepore Boston globin gene

M. Baird, H. Schreiner, C. Driscoll, A. Bank

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The site of crossover between the δ- and β-globin gene sequence resulting in Lepore Boston globin gene formation has been localized at the DNA level. Probes specific for detecting the intervening sequences (IVS) of the δ- and β-globin genes were used to map the origin of cellular DNA fragments of a patient homozygous for hemoglobin Lepore Boston. Restriction endonuclease analysis and hybridization of this DNA to IVS specific probes show that most, if not all, of the large intervening sequences (IVS 2) in Lepore DNA are derived from the β-globin gene IVS 2. In addition, the crossover occurs in a region of DNA in which the δ- and β-genes have almost complete nucleotide homology, and might be expected to pair most tightly during meiosis.

Original languageEnglish (US)
Pages (from-to)560-564
Number of pages5
JournalJournal of Clinical Investigation
Volume68
Issue number2
StatePublished - 1981
Externally publishedYes

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Globins
Introns
Genetic Recombination
DNA
Genes
DNA Restriction Enzymes
Meiosis
Nucleotides

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Localization of the site of recombination in formation of the lepore Boston globin gene. / Baird, M.; Schreiner, H.; Driscoll, C.; Bank, A.

In: Journal of Clinical Investigation, Vol. 68, No. 2, 1981, p. 560-564.

Research output: Contribution to journalArticle

Baird, M, Schreiner, H, Driscoll, C & Bank, A 1981, 'Localization of the site of recombination in formation of the lepore Boston globin gene', Journal of Clinical Investigation, vol. 68, no. 2, pp. 560-564.
Baird, M. ; Schreiner, H. ; Driscoll, C. ; Bank, A. / Localization of the site of recombination in formation of the lepore Boston globin gene. In: Journal of Clinical Investigation. 1981 ; Vol. 68, No. 2. pp. 560-564.
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