Localization of the human type 5, tartrate-resistant acid phosphatase gene by in situ hybridization

Beverly S. Allen; Catherine M. Ketcham; R. Michael Roberts; Harry S. Nick; Harry Ostrer

doi:10.1016/0888-7543(89)90284-X

Localization of the human type 5, tartrate-resistant acid phosphatase gene by in situ hybridization

Beverly S. Allen, Catherine M. Ketcham, R. Michael Roberts, Harry S. Nick, Harry Ostrer

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

We report the localization of the gene for the human type 5, tartrate-resistant, iron-containing acid phosphatase isoenzyme (HGM designation ACP5) to chromosome 15 (15q22-q26) using the technique of in situ hybridization to metaphase chromosomes. We have localized this gene using peripheral blood chromosomes obtained from both a normal male and an individual carrying an unbalanced translocation involving chromosome 15 [45,XY,-15,-18,+der(18)-t(15;18)(q13;p11)]. In addition, we have demonstrated the utility of employing a standard fluorescent staining technique (distamycin/DAPI) to an emulsion-coated, Wright-stained, and destained chromosome preparation.

Original language	English (US)
Pages (from-to)	597-600
Number of pages	4
Journal	Genomics
Volume	4
Issue number	4
DOIs	https://doi.org/10.1016/0888-7543(89)90284-X
State	Published - May 1989
Externally published	Yes

ASJC Scopus subject areas

Genetics

Access to Document

10.1016/0888-7543(89)90284-X

Fingerprint Dive into the research topics of 'Localization of the human type 5, tartrate-resistant acid phosphatase gene by in situ hybridization'. Together they form a unique fingerprint.

Cite this

@article{97148794a99946f2965ca3b68581e4c9,

title = "Localization of the human type 5, tartrate-resistant acid phosphatase gene by in situ hybridization",

abstract = "We report the localization of the gene for the human type 5, tartrate-resistant, iron-containing acid phosphatase isoenzyme (HGM designation ACP5) to chromosome 15 (15q22-q26) using the technique of in situ hybridization to metaphase chromosomes. We have localized this gene using peripheral blood chromosomes obtained from both a normal male and an individual carrying an unbalanced translocation involving chromosome 15 [45,XY,-15,-18,+der(18)-t(15;18)(q13;p11)]. In addition, we have demonstrated the utility of employing a standard fluorescent staining technique (distamycin/DAPI) to an emulsion-coated, Wright-stained, and destained chromosome preparation.",

author = "Allen, {Beverly S.} and Ketcham, {Catherine M.} and {Michael Roberts}, R. and Nick, {Harry S.} and Harry Ostrer",

note = "Funding Information: This work was supported in part by the National Institutes of Health HD21980 (R.M.R.) and a Basil O{\textquoteright}Connor Starter Grant (S-585) from the March of Dimes Birth Defects Foundation (H.O.) and by the State of Florida, Department of Health and Rehabilitative Services.",

year = "1989",

month = may,

doi = "10.1016/0888-7543(89)90284-X",

language = "English (US)",

volume = "4",

pages = "597--600",

journal = "Genomics",

issn = "0888-7543",

publisher = "Academic Press Inc.",

number = "4",

}

TY - JOUR

T1 - Localization of the human type 5, tartrate-resistant acid phosphatase gene by in situ hybridization

AU - Allen, Beverly S.

AU - Ketcham, Catherine M.

AU - Michael Roberts, R.

AU - Nick, Harry S.

AU - Ostrer, Harry

N1 - Funding Information: This work was supported in part by the National Institutes of Health HD21980 (R.M.R.) and a Basil O’Connor Starter Grant (S-585) from the March of Dimes Birth Defects Foundation (H.O.) and by the State of Florida, Department of Health and Rehabilitative Services.

PY - 1989/5

Y1 - 1989/5

N2 - We report the localization of the gene for the human type 5, tartrate-resistant, iron-containing acid phosphatase isoenzyme (HGM designation ACP5) to chromosome 15 (15q22-q26) using the technique of in situ hybridization to metaphase chromosomes. We have localized this gene using peripheral blood chromosomes obtained from both a normal male and an individual carrying an unbalanced translocation involving chromosome 15 [45,XY,-15,-18,+der(18)-t(15;18)(q13;p11)]. In addition, we have demonstrated the utility of employing a standard fluorescent staining technique (distamycin/DAPI) to an emulsion-coated, Wright-stained, and destained chromosome preparation.

AB - We report the localization of the gene for the human type 5, tartrate-resistant, iron-containing acid phosphatase isoenzyme (HGM designation ACP5) to chromosome 15 (15q22-q26) using the technique of in situ hybridization to metaphase chromosomes. We have localized this gene using peripheral blood chromosomes obtained from both a normal male and an individual carrying an unbalanced translocation involving chromosome 15 [45,XY,-15,-18,+der(18)-t(15;18)(q13;p11)]. In addition, we have demonstrated the utility of employing a standard fluorescent staining technique (distamycin/DAPI) to an emulsion-coated, Wright-stained, and destained chromosome preparation.

UR - http://www.scopus.com/inward/record.url?scp=0024669957&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024669957&partnerID=8YFLogxK

U2 - 10.1016/0888-7543(89)90284-X

DO - 10.1016/0888-7543(89)90284-X

M3 - Article

C2 - 2473026

AN - SCOPUS:0024669957

VL - 4

SP - 597

EP - 600

JO - Genomics

JF - Genomics

SN - 0888-7543

IS - 4

ER -