Abstract
We report the localization of the gene for the human type 5, tartrate-resistant, iron-containing acid phosphatase isoenzyme (HGM designation ACP5) to chromosome 15 (15q22-q26) using the technique of in situ hybridization to metaphase chromosomes. We have localized this gene using peripheral blood chromosomes obtained from both a normal male and an individual carrying an unbalanced translocation involving chromosome 15 [45,XY,-15,-18,+der(18)-t(15;18)(q13;p11)]. In addition, we have demonstrated the utility of employing a standard fluorescent staining technique (distamycin/DAPI) to an emulsion-coated, Wright-stained, and destained chromosome preparation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 597-600 |
| Number of pages | 4 |
| Journal | Genomics |
| Volume | 4 |
| Issue number | 4 |
| DOIs | |
| State | Published - 1989 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Genetics
Cite this
Localization of the human type 5, tartrate-resistant acid phosphatase gene by in situ hybridization. / Allen, Beverly S.; Ketcham, Catherine M.; Michael Roberts, R.; Nick, Harry S.; Ostrer, Harry.
In: Genomics, Vol. 4, No. 4, 1989, p. 597-600.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Localization of the human type 5, tartrate-resistant acid phosphatase gene by in situ hybridization
AU - Allen, Beverly S.
AU - Ketcham, Catherine M.
AU - Michael Roberts, R.
AU - Nick, Harry S.
AU - Ostrer, Harry
PY - 1989
Y1 - 1989
N2 - We report the localization of the gene for the human type 5, tartrate-resistant, iron-containing acid phosphatase isoenzyme (HGM designation ACP5) to chromosome 15 (15q22-q26) using the technique of in situ hybridization to metaphase chromosomes. We have localized this gene using peripheral blood chromosomes obtained from both a normal male and an individual carrying an unbalanced translocation involving chromosome 15 [45,XY,-15,-18,+der(18)-t(15;18)(q13;p11)]. In addition, we have demonstrated the utility of employing a standard fluorescent staining technique (distamycin/DAPI) to an emulsion-coated, Wright-stained, and destained chromosome preparation.
AB - We report the localization of the gene for the human type 5, tartrate-resistant, iron-containing acid phosphatase isoenzyme (HGM designation ACP5) to chromosome 15 (15q22-q26) using the technique of in situ hybridization to metaphase chromosomes. We have localized this gene using peripheral blood chromosomes obtained from both a normal male and an individual carrying an unbalanced translocation involving chromosome 15 [45,XY,-15,-18,+der(18)-t(15;18)(q13;p11)]. In addition, we have demonstrated the utility of employing a standard fluorescent staining technique (distamycin/DAPI) to an emulsion-coated, Wright-stained, and destained chromosome preparation.
UR - http://www.scopus.com/inward/record.url?scp=0024669957&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024669957&partnerID=8YFLogxK
U2 - 10.1016/0888-7543(89)90284-X
DO - 10.1016/0888-7543(89)90284-X
M3 - Article
VL - 4
SP - 597
EP - 600
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 4
ER -