TY - JOUR
T1 - Localization of the human type 5, tartrate-resistant acid phosphatase gene by in situ hybridization
AU - Allen, Beverly S.
AU - Ketcham, Catherine M.
AU - Michael Roberts, R.
AU - Nick, Harry S.
AU - Ostrer, Harry
N1 - Funding Information:
This work was supported in part by the National Institutes of Health HD21980 (R.M.R.) and a Basil O’Connor Starter Grant (S-585) from the March of Dimes Birth Defects Foundation (H.O.) and by the State of Florida, Department of Health and Rehabilitative Services.
PY - 1989/5
Y1 - 1989/5
N2 - We report the localization of the gene for the human type 5, tartrate-resistant, iron-containing acid phosphatase isoenzyme (HGM designation ACP5) to chromosome 15 (15q22-q26) using the technique of in situ hybridization to metaphase chromosomes. We have localized this gene using peripheral blood chromosomes obtained from both a normal male and an individual carrying an unbalanced translocation involving chromosome 15 [45,XY,-15,-18,+der(18)-t(15;18)(q13;p11)]. In addition, we have demonstrated the utility of employing a standard fluorescent staining technique (distamycin/DAPI) to an emulsion-coated, Wright-stained, and destained chromosome preparation.
AB - We report the localization of the gene for the human type 5, tartrate-resistant, iron-containing acid phosphatase isoenzyme (HGM designation ACP5) to chromosome 15 (15q22-q26) using the technique of in situ hybridization to metaphase chromosomes. We have localized this gene using peripheral blood chromosomes obtained from both a normal male and an individual carrying an unbalanced translocation involving chromosome 15 [45,XY,-15,-18,+der(18)-t(15;18)(q13;p11)]. In addition, we have demonstrated the utility of employing a standard fluorescent staining technique (distamycin/DAPI) to an emulsion-coated, Wright-stained, and destained chromosome preparation.
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U2 - 10.1016/0888-7543(89)90284-X
DO - 10.1016/0888-7543(89)90284-X
M3 - Article
C2 - 2473026
AN - SCOPUS:0024669957
VL - 4
SP - 597
EP - 600
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 4
ER -