Localization of the human type 5, tartrate-resistant acid phosphatase gene by in situ hybridization

Beverly S. Allen; Catherine M. Ketcham; R. Michael Roberts; Harry S. Nick; Harry Ostrer

doi:10.1016/0888-7543(89)90284-X

Localization of the human type 5, tartrate-resistant acid phosphatase gene by in situ hybridization

Beverly S. Allen, Catherine M. Ketcham, R. Michael Roberts, Harry S. Nick, Harry Ostrer

Research output: Contribution to journal › Article

10 Scopus citations

Abstract

We report the localization of the gene for the human type 5, tartrate-resistant, iron-containing acid phosphatase isoenzyme (HGM designation ACP5) to chromosome 15 (15q22-q26) using the technique of in situ hybridization to metaphase chromosomes. We have localized this gene using peripheral blood chromosomes obtained from both a normal male and an individual carrying an unbalanced translocation involving chromosome 15 [45,XY,-15,-18,+der(18)-t(15;18)(q13;p11)]. In addition, we have demonstrated the utility of employing a standard fluorescent staining technique (distamycin/DAPI) to an emulsion-coated, Wright-stained, and destained chromosome preparation.

Original language	English (US)
Pages (from-to)	597-600
Number of pages	4
Journal	Genomics
Volume	4
Issue number	4
DOIs	https://doi.org/10.1016/0888-7543(89)90284-X
State	Published - May 1989
Externally published	Yes

ASJC Scopus subject areas

Genetics

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Allen, B. S., Ketcham, C. M., Michael Roberts, R., Nick, H. S., & Ostrer, H. (1989). Localization of the human type 5, tartrate-resistant acid phosphatase gene by in situ hybridization. Genomics, 4(4), 597-600. https://doi.org/10.1016/0888-7543(89)90284-X

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abstract = "We report the localization of the gene for the human type 5, tartrate-resistant, iron-containing acid phosphatase isoenzyme (HGM designation ACP5) to chromosome 15 (15q22-q26) using the technique of in situ hybridization to metaphase chromosomes. We have localized this gene using peripheral blood chromosomes obtained from both a normal male and an individual carrying an unbalanced translocation involving chromosome 15 [45,XY,-15,-18,+der(18)-t(15;18)(q13;p11)]. In addition, we have demonstrated the utility of employing a standard fluorescent staining technique (distamycin/DAPI) to an emulsion-coated, Wright-stained, and destained chromosome preparation.",

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AU - Nick, Harry S.

AU - Ostrer, Harry

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