Localization of the human type 5, tartrate-resistant acid phosphatase gene by in situ hybridization

Beverly S. Allen, Catherine M. Ketcham, R. Michael Roberts, Harry S. Nick, Harry Ostrer

Research output: Contribution to journalArticle

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Abstract

We report the localization of the gene for the human type 5, tartrate-resistant, iron-containing acid phosphatase isoenzyme (HGM designation ACP5) to chromosome 15 (15q22-q26) using the technique of in situ hybridization to metaphase chromosomes. We have localized this gene using peripheral blood chromosomes obtained from both a normal male and an individual carrying an unbalanced translocation involving chromosome 15 [45,XY,-15,-18,+der(18)-t(15;18)(q13;p11)]. In addition, we have demonstrated the utility of employing a standard fluorescent staining technique (distamycin/DAPI) to an emulsion-coated, Wright-stained, and destained chromosome preparation.

Original languageEnglish (US)
Pages (from-to)597-600
Number of pages4
JournalGenomics
Volume4
Issue number4
DOIs
Publication statusPublished - May 1989
Externally publishedYes

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ASJC Scopus subject areas

  • Genetics

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