Localization of the human type 5, tartrate-resistant acid phosphatase gene by in situ hybridization

Beverly S. Allen, Catherine M. Ketcham, R. Michael Roberts, Harry S. Nick, Harry Ostrer

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We report the localization of the gene for the human type 5, tartrate-resistant, iron-containing acid phosphatase isoenzyme (HGM designation ACP5) to chromosome 15 (15q22-q26) using the technique of in situ hybridization to metaphase chromosomes. We have localized this gene using peripheral blood chromosomes obtained from both a normal male and an individual carrying an unbalanced translocation involving chromosome 15 [45,XY,-15,-18,+der(18)-t(15;18)(q13;p11)]. In addition, we have demonstrated the utility of employing a standard fluorescent staining technique (distamycin/DAPI) to an emulsion-coated, Wright-stained, and destained chromosome preparation.

Original languageEnglish (US)
Pages (from-to)597-600
Number of pages4
JournalGenomics
Volume4
Issue number4
DOIs
StatePublished - 1989
Externally publishedYes

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In Situ Hybridization
Chromosomes, Human, Pair 15
Chromosomes
Genes
Metaphase
Acid Phosphatase
Emulsions
Isoenzymes
Iron
Staining and Labeling
Tartrate-Resistant Acid Phosphatase
tartaric acid
distamycin-DAPI

ASJC Scopus subject areas

  • Genetics

Cite this

Localization of the human type 5, tartrate-resistant acid phosphatase gene by in situ hybridization. / Allen, Beverly S.; Ketcham, Catherine M.; Michael Roberts, R.; Nick, Harry S.; Ostrer, Harry.

In: Genomics, Vol. 4, No. 4, 1989, p. 597-600.

Research output: Contribution to journalArticle

Allen, Beverly S. ; Ketcham, Catherine M. ; Michael Roberts, R. ; Nick, Harry S. ; Ostrer, Harry. / Localization of the human type 5, tartrate-resistant acid phosphatase gene by in situ hybridization. In: Genomics. 1989 ; Vol. 4, No. 4. pp. 597-600.
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