Localization of septin proteins in the mouse cochlea

Atsuhiro Yoshida, Norio Yamamoto, Makoto Kinoshita, Noboru Hiroi, Takeshi Hiramoto, Gina Kang, William S. Trimble, Kenji Tanigaki, Takayuki Nakagawa, Juichi Ito

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Septins are a family of GTP binding proteins that are well conserved in eukaryotic species except plants. Septins contribute to the lateral compartmentalization of membranes, cortical rigidity, and the regulation of membrane trafficking by associating with membrane lipids, actin, and microtubules. The organ of Corti in the cochlea has pivotal roles in auditory perception and includes two kinds of highly polarized cells, hair and supporting cells, both of which are rich in actin and microtubules. To identify the roles of septins in the cochlea, we analyzed the localization of three septin proteins, septin 4 (SEPT4), septin 5 (SEPT5), and septin 7 (SEPT7) that are abundantly expressed in brain tissues, and also examined auditory functions of . Sept4 and . Sept5 null mice. SEPT4, SEPT5, and SEPT7 were expressed in inner and outer pillar cells and Deiters' cells but the distribution patterns of each protein in Deiters' cells were different. SEPT4 and SEPT7 were expressed in the phalangeal process where SEPT5 was not detected. In addition to these cells SEPT5 and SEPT7 were co-localized with presynaptic vesicles of efferent nerve terminals. Only SEPT7 was expressed in the cochlea at embryonic stages. Although expression patterns of septin proteins suggested their important roles in the function of the cochlea, both . Sept4 and . Sept5 null mice had similar auditory functions to their wild type littermates. Immunohistochemical analysis of . Sept4 null mice showed that compensatory expression of SEPT5 in the phalangeal process of Deiters' cells may have caused functional compensation of hearing ability in . Sept4 null mice.

Original languageEnglish (US)
Pages (from-to)40-51
Number of pages12
JournalHearing Research
Volume289
Issue number1-2
DOIs
StatePublished - Jul 2012

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Septins
Cochlea
Proteins
Microtubules
Actins
Auditory Perception

ASJC Scopus subject areas

  • Sensory Systems

Cite this

Yoshida, A., Yamamoto, N., Kinoshita, M., Hiroi, N., Hiramoto, T., Kang, G., ... Ito, J. (2012). Localization of septin proteins in the mouse cochlea. Hearing Research, 289(1-2), 40-51. https://doi.org/10.1016/j.heares.2012.04.015

Localization of septin proteins in the mouse cochlea. / Yoshida, Atsuhiro; Yamamoto, Norio; Kinoshita, Makoto; Hiroi, Noboru; Hiramoto, Takeshi; Kang, Gina; Trimble, William S.; Tanigaki, Kenji; Nakagawa, Takayuki; Ito, Juichi.

In: Hearing Research, Vol. 289, No. 1-2, 07.2012, p. 40-51.

Research output: Contribution to journalArticle

Yoshida, A, Yamamoto, N, Kinoshita, M, Hiroi, N, Hiramoto, T, Kang, G, Trimble, WS, Tanigaki, K, Nakagawa, T & Ito, J 2012, 'Localization of septin proteins in the mouse cochlea', Hearing Research, vol. 289, no. 1-2, pp. 40-51. https://doi.org/10.1016/j.heares.2012.04.015
Yoshida A, Yamamoto N, Kinoshita M, Hiroi N, Hiramoto T, Kang G et al. Localization of septin proteins in the mouse cochlea. Hearing Research. 2012 Jul;289(1-2):40-51. https://doi.org/10.1016/j.heares.2012.04.015
Yoshida, Atsuhiro ; Yamamoto, Norio ; Kinoshita, Makoto ; Hiroi, Noboru ; Hiramoto, Takeshi ; Kang, Gina ; Trimble, William S. ; Tanigaki, Kenji ; Nakagawa, Takayuki ; Ito, Juichi. / Localization of septin proteins in the mouse cochlea. In: Hearing Research. 2012 ; Vol. 289, No. 1-2. pp. 40-51.
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