Localization of plasma membrane and secretory calcium pumps in the mammary gland

Helen M. Faddy, Chanel E. Smart, Ren Xu, Genee Y. Lee, Paraic A. Kenny, Mingye Feng, Rajini Rao, Melissa A. Brown, Mina J. Bissell, Sarah J. Roberts-Thomson, Gregory R. Monteith

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Until recently the mechanism for the enrichment of milk with calcium was thought to be almost entirely via the secretory pathway. However, recent studies suggest that a plasma membrane calcium ATPase, PMCA2, is the primary mechanism for calcium transport into milk, highlighting a major role for apical calcium transport. We compared the expression of the recently identified secretory calcium ATPase, SPCA2, and SPCA1, in the mouse mammary gland during development. SPCA2 levels increased over 35-fold during lactation with expression localized to luminal secretory cells, while SPCA1 increased only a modest 2-fold and was expressed throughout the cells of the mammary gland. We also observed major differences in the localization of PMCA2 and PMCA1. Our studies highlight the likely specific roles of PMCA2 and SPCA2 in lactation and indicate that calcium transport into milk is a complex interplay between apical and secretory pathways.

Original languageEnglish (US)
Pages (from-to)977-981
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume369
Issue number3
DOIs
StatePublished - May 9 2008
Externally publishedYes

Fingerprint

Cell membranes
Human Mammary Glands
Secretory Pathway
Cell Membrane
Pumps
Calcium
Lactation
Plasma Membrane Calcium-Transporting ATPases
Milk
Calcium-Transporting ATPases
Calcium Carbonate

Keywords

  • 3D mammary culture
  • Calcium
  • Mammary gland
  • Milk
  • Mouse
  • PMCA1
  • PMCA2
  • SPCA1
  • SPCA2

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Faddy, H. M., Smart, C. E., Xu, R., Lee, G. Y., Kenny, P. A., Feng, M., ... Monteith, G. R. (2008). Localization of plasma membrane and secretory calcium pumps in the mammary gland. Biochemical and Biophysical Research Communications, 369(3), 977-981. https://doi.org/10.1016/j.bbrc.2008.03.003

Localization of plasma membrane and secretory calcium pumps in the mammary gland. / Faddy, Helen M.; Smart, Chanel E.; Xu, Ren; Lee, Genee Y.; Kenny, Paraic A.; Feng, Mingye; Rao, Rajini; Brown, Melissa A.; Bissell, Mina J.; Roberts-Thomson, Sarah J.; Monteith, Gregory R.

In: Biochemical and Biophysical Research Communications, Vol. 369, No. 3, 09.05.2008, p. 977-981.

Research output: Contribution to journalArticle

Faddy, HM, Smart, CE, Xu, R, Lee, GY, Kenny, PA, Feng, M, Rao, R, Brown, MA, Bissell, MJ, Roberts-Thomson, SJ & Monteith, GR 2008, 'Localization of plasma membrane and secretory calcium pumps in the mammary gland', Biochemical and Biophysical Research Communications, vol. 369, no. 3, pp. 977-981. https://doi.org/10.1016/j.bbrc.2008.03.003
Faddy, Helen M. ; Smart, Chanel E. ; Xu, Ren ; Lee, Genee Y. ; Kenny, Paraic A. ; Feng, Mingye ; Rao, Rajini ; Brown, Melissa A. ; Bissell, Mina J. ; Roberts-Thomson, Sarah J. ; Monteith, Gregory R. / Localization of plasma membrane and secretory calcium pumps in the mammary gland. In: Biochemical and Biophysical Research Communications. 2008 ; Vol. 369, No. 3. pp. 977-981.
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