Abstract
The sequence of the mRNAs which encode the α-subunits of the signal-transducing G-proteins Gs, Go and two forms of Gi (termed Gi1 and Gi2) have recently been reported. Based on rat sequences we prepared oligodeoxynucleotide probes for measurement of these mRNAs in rat brain and peripheral tissues. The relative abundance of these mRNA species in brain was Gs > Go ∼ Gi2 > Gi1. The Gs and Gi2 mRNAs had somewhat lower levels in heart, kidney and liver than in brain, and Go and Gi1 mRNAs were not detected in the peripheral tissues. Using in situ hybridization we localized each of these mRNAs within slices of the rat brain. The patterns of distribution of Gs and Gi2 mRNA were very similar, but very different from that of Go and Gi1 mRNA. These data illustrate that receptor-effector coupling G-proteins are regionally specialized in their expression. This regional specialization may reflect a selective coupling of individual G-proteins with the various neurotransmitter receptors and effector pathways.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 191-198 |
| Number of pages | 8 |
| Journal | FEBS Letters |
| Volume | 222 |
| Issue number | 1 |
| DOIs | |
| State | Published - Sep 28 1987 |
| Externally published | Yes |
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Keywords
- G-protein
- Hybridization
- mRNA
- Neurotransmitter receptor
- Signal transduction
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Localization of mRNAs encoding the α-subunits of signal-transducing G-proteins within rat brain and among peripheral tissues. / Brann, Mark R.; Collins, Regina M.; Spiegel, Allen M.
In: FEBS Letters, Vol. 222, No. 1, 28.09.1987, p. 191-198.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Localization of mRNAs encoding the α-subunits of signal-transducing G-proteins within rat brain and among peripheral tissues
AU - Brann, Mark R.
AU - Collins, Regina M.
AU - Spiegel, Allen M.
PY - 1987/9/28
Y1 - 1987/9/28
N2 - The sequence of the mRNAs which encode the α-subunits of the signal-transducing G-proteins Gs, Go and two forms of Gi (termed Gi1 and Gi2) have recently been reported. Based on rat sequences we prepared oligodeoxynucleotide probes for measurement of these mRNAs in rat brain and peripheral tissues. The relative abundance of these mRNA species in brain was Gs > Go ∼ Gi2 > Gi1. The Gs and Gi2 mRNAs had somewhat lower levels in heart, kidney and liver than in brain, and Go and Gi1 mRNAs were not detected in the peripheral tissues. Using in situ hybridization we localized each of these mRNAs within slices of the rat brain. The patterns of distribution of Gs and Gi2 mRNA were very similar, but very different from that of Go and Gi1 mRNA. These data illustrate that receptor-effector coupling G-proteins are regionally specialized in their expression. This regional specialization may reflect a selective coupling of individual G-proteins with the various neurotransmitter receptors and effector pathways.
AB - The sequence of the mRNAs which encode the α-subunits of the signal-transducing G-proteins Gs, Go and two forms of Gi (termed Gi1 and Gi2) have recently been reported. Based on rat sequences we prepared oligodeoxynucleotide probes for measurement of these mRNAs in rat brain and peripheral tissues. The relative abundance of these mRNA species in brain was Gs > Go ∼ Gi2 > Gi1. The Gs and Gi2 mRNAs had somewhat lower levels in heart, kidney and liver than in brain, and Go and Gi1 mRNAs were not detected in the peripheral tissues. Using in situ hybridization we localized each of these mRNAs within slices of the rat brain. The patterns of distribution of Gs and Gi2 mRNA were very similar, but very different from that of Go and Gi1 mRNA. These data illustrate that receptor-effector coupling G-proteins are regionally specialized in their expression. This regional specialization may reflect a selective coupling of individual G-proteins with the various neurotransmitter receptors and effector pathways.
KW - G-protein
KW - Hybridization
KW - mRNA
KW - Neurotransmitter receptor
KW - Signal transduction
UR - http://www.scopus.com/inward/record.url?scp=0023667133&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023667133&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(87)80218-1
DO - 10.1016/0014-5793(87)80218-1
M3 - Article
C2 - 3115828
AN - SCOPUS:0023667133
VL - 222
SP - 191
EP - 198
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 1
ER -