Localization of mRNAs encoding the α-subunits of signal-transducing G-proteins within rat brain and among peripheral tissues

Mark R. Brann, Regina M. Collins, Allen M. Spiegel

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

The sequence of the mRNAs which encode the α-subunits of the signal-transducing G-proteins Gs, Go and two forms of Gi (termed Gi1 and Gi2) have recently been reported. Based on rat sequences we prepared oligodeoxynucleotide probes for measurement of these mRNAs in rat brain and peripheral tissues. The relative abundance of these mRNA species in brain was Gs > Go ∼ Gi2 > Gi1. The Gs and Gi2 mRNAs had somewhat lower levels in heart, kidney and liver than in brain, and Go and Gi1 mRNAs were not detected in the peripheral tissues. Using in situ hybridization we localized each of these mRNAs within slices of the rat brain. The patterns of distribution of Gs and Gi2 mRNA were very similar, but very different from that of Go and Gi1 mRNA. These data illustrate that receptor-effector coupling G-proteins are regionally specialized in their expression. This regional specialization may reflect a selective coupling of individual G-proteins with the various neurotransmitter receptors and effector pathways.

Original languageEnglish (US)
Pages (from-to)191-198
Number of pages8
JournalFEBS Letters
Volume222
Issue number1
DOIs
StatePublished - Sep 28 1987
Externally publishedYes

Fingerprint

GTP-Binding Proteins
Rats
Brain
Tissue
Messenger RNA
Gi-Go GTP-Binding Protein alpha Subunits
Neurotransmitter Receptor
Oligodeoxyribonucleotides
Liver
In Situ Hybridization
Kidney

Keywords

  • G-protein
  • Hybridization
  • mRNA
  • Neurotransmitter receptor
  • Signal transduction

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Localization of mRNAs encoding the α-subunits of signal-transducing G-proteins within rat brain and among peripheral tissues. / Brann, Mark R.; Collins, Regina M.; Spiegel, Allen M.

In: FEBS Letters, Vol. 222, No. 1, 28.09.1987, p. 191-198.

Research output: Contribution to journalArticle

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