Localization of ASH1 mRNA particles in living yeast

Edouard Bertrand; Pascal Chartrand; Matthias Schaefer; Shailesh M. Shenoy; Robert H. Singer; Roy M. Long

doi:10.1016/S1097-2765(00)80143-4

Localization of ASH1 mRNA particles in living yeast

Edouard Bertrand, Pascal Chartrand, Matthias Schaefer, Shailesh M. Shenoy, Robert H. Singer, Roy M. Long

Cell Biology

Research output: Contribution to journal › Article › peer-review

1243 Scopus citations

Abstract

ASH1 mRNA localizes to the bud tip in Saccharomyces cerevisiae to establish asymmetry of HO expression, important for mating type switching. To visualize real time localization of the mRNA in living yeast cells, green fluorescent protein (GFP) was fused to the RNA-binding protein MS2 to follow a reporter mRNA containing MS2-binding sites. Formation and localization of a GFP particle in the bud required ASH1 3′UTR (untranslated region) sequences. The SHE mutants disrupt RNA and particle localization and SHE 2 and 3 mutants inhibit particle formation as well. Both She3myc and Shelmyc colocalized with the particle. Video microscopy demonstrated that She1p/Myo4p moved particles to the bud tip at 200-440 nm/sec. Therefore, the ASH1 3′UTR-dependent particle serves as a marker for RNA transport and localization.

Original language	English (US)
Pages (from-to)	437-445
Number of pages	9
Journal	Molecular Cell
Volume	2
Issue number	4
DOIs	https://doi.org/10.1016/S1097-2765(00)80143-4
State	Published - Oct 1998

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1016/S1097-2765(00)80143-4

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title = "Localization of ASH1 mRNA particles in living yeast",

abstract = "ASH1 mRNA localizes to the bud tip in Saccharomyces cerevisiae to establish asymmetry of HO expression, important for mating type switching. To visualize real time localization of the mRNA in living yeast cells, green fluorescent protein (GFP) was fused to the RNA-binding protein MS2 to follow a reporter mRNA containing MS2-binding sites. Formation and localization of a GFP particle in the bud required ASH1 3′UTR (untranslated region) sequences. The SHE mutants disrupt RNA and particle localization and SHE 2 and 3 mutants inhibit particle formation as well. Both She3myc and Shelmyc colocalized with the particle. Video microscopy demonstrated that She1p/Myo4p moved particles to the bud tip at 200-440 nm/sec. Therefore, the ASH1 3′UTR-dependent particle serves as a marker for RNA transport and localization.",

author = "Edouard Bertrand and Pascal Chartrand and Matthias Schaefer and Shenoy, {Shailesh M.} and Singer, {Robert H.} and Long, {Roy M.}",

note = "Funding Information: Supported by GM 54887 and GM 57071 to R.H.S., Canadian Fonds pour la Formation de Chercheurs et l{\textquoteright}Aide {\`a} la Recherche fellowship to P.C., and F32-HD08088 to R.M.L. The authors thank Michael Rosbash and John Warner for helpful discussions.",

year = "1998",

month = oct,

doi = "10.1016/S1097-2765(00)80143-4",

language = "English (US)",

volume = "2",

pages = "437--445",

journal = "Molecular Cell",

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T1 - Localization of ASH1 mRNA particles in living yeast

AU - Bertrand, Edouard

AU - Chartrand, Pascal

AU - Schaefer, Matthias

AU - Shenoy, Shailesh M.

AU - Singer, Robert H.

AU - Long, Roy M.

N1 - Funding Information: Supported by GM 54887 and GM 57071 to R.H.S., Canadian Fonds pour la Formation de Chercheurs et l’Aide à la Recherche fellowship to P.C., and F32-HD08088 to R.M.L. The authors thank Michael Rosbash and John Warner for helpful discussions.

PY - 1998/10

Y1 - 1998/10

N2 - ASH1 mRNA localizes to the bud tip in Saccharomyces cerevisiae to establish asymmetry of HO expression, important for mating type switching. To visualize real time localization of the mRNA in living yeast cells, green fluorescent protein (GFP) was fused to the RNA-binding protein MS2 to follow a reporter mRNA containing MS2-binding sites. Formation and localization of a GFP particle in the bud required ASH1 3′UTR (untranslated region) sequences. The SHE mutants disrupt RNA and particle localization and SHE 2 and 3 mutants inhibit particle formation as well. Both She3myc and Shelmyc colocalized with the particle. Video microscopy demonstrated that She1p/Myo4p moved particles to the bud tip at 200-440 nm/sec. Therefore, the ASH1 3′UTR-dependent particle serves as a marker for RNA transport and localization.

AB - ASH1 mRNA localizes to the bud tip in Saccharomyces cerevisiae to establish asymmetry of HO expression, important for mating type switching. To visualize real time localization of the mRNA in living yeast cells, green fluorescent protein (GFP) was fused to the RNA-binding protein MS2 to follow a reporter mRNA containing MS2-binding sites. Formation and localization of a GFP particle in the bud required ASH1 3′UTR (untranslated region) sequences. The SHE mutants disrupt RNA and particle localization and SHE 2 and 3 mutants inhibit particle formation as well. Both She3myc and Shelmyc colocalized with the particle. Video microscopy demonstrated that She1p/Myo4p moved particles to the bud tip at 200-440 nm/sec. Therefore, the ASH1 3′UTR-dependent particle serves as a marker for RNA transport and localization.

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AN - SCOPUS:0032185810

SN - 1097-2765

VL - 2

SP - 437

EP - 445

JO - Molecular Cell

JF - Molecular Cell

IS - 4

ER -

Localization of ASH1 mRNA particles in living yeast

Abstract

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