Localization of ASH1 mRNA particles in living yeast

Edouard Bertrand, Pascal Chartrand, Matthias Schaefer, Shailesh M. Shenoy, Robert H. Singer, Roy M. Long

Research output: Contribution to journalArticle

884 Citations (Scopus)

Abstract

ASH1 mRNA localizes to the bud tip in Saccharomyces cerevisiae to establish asymmetry of HO expression, important for mating type switching. To visualize real time localization of the mRNA in living yeast cells, green fluorescent protein (GFP) was fused to the RNA-binding protein MS2 to follow a reporter mRNA containing MS2-binding sites. Formation and localization of a GFP particle in the bud required ASH1 3′UTR (untranslated region) sequences. The SHE mutants disrupt RNA and particle localization and SHE 2 and 3 mutants inhibit particle formation as well. Both She3myc and Shelmyc colocalized with the particle. Video microscopy demonstrated that She1p/Myo4p moved particles to the bud tip at 200-440 nm/sec. Therefore, the ASH1 3′UTR-dependent particle serves as a marker for RNA transport and localization.

Original languageEnglish (US)
Pages (from-to)437-445
Number of pages9
JournalMolecular Cell
Volume2
Issue number4
StatePublished - Oct 1998

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Yeasts
Green Fluorescent Proteins
Messenger RNA
RNA Transport
Untranslated Regions
Video Microscopy
RNA-Binding Proteins
Saccharomyces cerevisiae
Binding Sites
RNA

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Bertrand, E., Chartrand, P., Schaefer, M., Shenoy, S. M., Singer, R. H., & Long, R. M. (1998). Localization of ASH1 mRNA particles in living yeast. Molecular Cell, 2(4), 437-445.

Localization of ASH1 mRNA particles in living yeast. / Bertrand, Edouard; Chartrand, Pascal; Schaefer, Matthias; Shenoy, Shailesh M.; Singer, Robert H.; Long, Roy M.

In: Molecular Cell, Vol. 2, No. 4, 10.1998, p. 437-445.

Research output: Contribution to journalArticle

Bertrand, E, Chartrand, P, Schaefer, M, Shenoy, SM, Singer, RH & Long, RM 1998, 'Localization of ASH1 mRNA particles in living yeast', Molecular Cell, vol. 2, no. 4, pp. 437-445.
Bertrand E, Chartrand P, Schaefer M, Shenoy SM, Singer RH, Long RM. Localization of ASH1 mRNA particles in living yeast. Molecular Cell. 1998 Oct;2(4):437-445.
Bertrand, Edouard ; Chartrand, Pascal ; Schaefer, Matthias ; Shenoy, Shailesh M. ; Singer, Robert H. ; Long, Roy M. / Localization of ASH1 mRNA particles in living yeast. In: Molecular Cell. 1998 ; Vol. 2, No. 4. pp. 437-445.
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