Localization of all seven messenger RNAs for the actin-polymerization nucleator Arp2/3 complex in the protrusions of fibroblasts

Lisa A. Mingle, Nataly N. Okuhama, Jian Shi, Robert H. Singer, John S. Condeelis, Gang Liu

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

The actin-related protein 2/3 (Arp2/3) complex is a crucial actin polymerization nucleator and is localized to the leading protrusions of migrating cells. However, how the multiprotein complex is targeted to the protrusions remains unknown. Here, we demonstrate that mRNAs for the seven subunits of the Arp2/3 complex are localized to the protrusions in fibroblasts, supporting a hypothesis that the Arp2/3 complex is targeted to its site of function by mRNA localization. Depletion of serum from culture medium inhibits Arp2/3-complex mRNA localization to the protrusion, whereas serum stimulation leads to significant mRNA localization within 30 minutes. The effect of serum suggests that Arp2/3-complex mRNA localization is a cellular response to extracellular stimuli. The localization of the Arp2/3 complex mRNAs is dependent on both actin filaments and microtubules, because disruption of either cytoskeletal system (with cytochalasin D and colchicine, respectively) inhibited the localization of all seven subunit mRNAs. In addition, myosin inhibitors significantly inhibit Arp2 mRNA localization in chicken embryo fibroblasts, suggesting a myosin motor dependent mechanism for Arp2/3-complex mRNA localization.

Original languageEnglish (US)
Pages (from-to)2425-2433
Number of pages9
JournalJournal of Cell Science
Volume118
Issue number11
DOIs
StatePublished - Jun 1 2005

Fingerprint

Actin-Related Protein 2-3 Complex
Polymerization
Actins
Fibroblasts
Messenger RNA
Myosins
Serum
Cytochalasin D
Multiprotein Complexes
Colchicine
Actin Cytoskeleton
Microtubules
Culture Media
Chickens
Embryonic Structures

Keywords

  • Actin cytoskeleton
  • Cell motility
  • Microtubules
  • mRNA targeting
  • Myosin

ASJC Scopus subject areas

  • Cell Biology

Cite this

Localization of all seven messenger RNAs for the actin-polymerization nucleator Arp2/3 complex in the protrusions of fibroblasts. / Mingle, Lisa A.; Okuhama, Nataly N.; Shi, Jian; Singer, Robert H.; Condeelis, John S.; Liu, Gang.

In: Journal of Cell Science, Vol. 118, No. 11, 01.06.2005, p. 2425-2433.

Research output: Contribution to journalArticle

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