Local signaling by the EGF receptor

Stephan J. Kempiak, Shu Chin Yip, Jonathan M. Backer, Jeffrey E. Segall

Research output: Contribution to journalArticle

36 Scopus citations

Abstract

Differing spatial scales of signaling cascades are critical for cell orientation during chemotactic responses. We used biotin EGF bound to streptavidin-coupled magnetic beads to locally stimulate cells overexpressing the EGF receptor. We have found that EGF-induced actin polymerization remains localized even under conditions of receptor overexpression. Conversely, EGF-induced ERK activation spreads throughout the cell body after EGF bead stimulation. The localized actin polymerization is independent of PI3-kinase and rho protein activity and requires Arp2/3 complex and cofilin function. Thus, we find differing spatial scales of signaling from the EGF receptor, supporting models of chemotaxis that integrate short- and long-range signaling.

Original languageEnglish (US)
Pages (from-to)781-787
Number of pages7
JournalJournal of Cell Biology
Volume162
Issue number5
DOIs
StatePublished - Sep 1 2003

Keywords

  • Chemotaxis
  • EGF bead
  • Epidermal growth factor
  • Receptor
  • Signal transduction

ASJC Scopus subject areas

  • Cell Biology

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