Listeria monocytogenes virulence factors, including listeriolysin O, are secreted in biologically active extracellular vesicles

Carolina Coelho, Lisa Brown, Maria Maryam, Raghav Vij, Daniel F.Q. Smith, Meagan C. Burnet, Jennifer E. Kyle, Heino M. Heyman, Jasmine Ramirez, Rafael Prados-Rosales, Gregoire Lauvau, Ernesto S. Nakayasu, Nathan R. Brady, Anne Hamacher-Brady, Isabelle Coppens, Arturo Casadevall

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Outer membrane vesicles produced by Gram-negative bacteria have been studied for half a century but the possibility that Gram-positive bacteria secrete extracellular vesicles (EVs) was not pursued until recently due to the assumption that the thick peptidoglycan cell wall would prevent their release to the environment. However, following their discovery in fungi, which also have cell walls, EVs have now been described for a variety of Gram-positive bacteria. EVs purified from Gram-positive bacteria are implicated in virulence, toxin release, and transference to host cells, eliciting immune responses, and spread of antibiotic resistance. Listeria monocytogenes is a Gram-positive bacterium that causes listeriosis. Here we report that L. monocytogenes produces EVs with diameters ranging from 20 to 200 nm, containing the pore-forming toxin listeriolysin O (LLO) and phosphatidylinositol-specific phospholipase C (PI-PLC). Cell-free EV preparations were toxic to mammalian cells, the murine macrophage cell line J774.16, in a LLO-dependent manner, evidencing EV biological activity. The deletion of plcA increased EV toxicity, suggesting PI-PLC reduced LLO activity. Using simultaneous metabolite, protein, and lipid extraction (MPLEx) multiomics we characterized protein, lipid, and metabolite composition of bacterial cells and secreted EVs and found that EVs carry the majority of listerial virulence proteins. Using immunogold EM we detected LLO at several organelles within infected human epithelial cells and with high-resolution fluorescence imaging we show that dynamic lipid structures are released from L. monocytogenes during infection. Our findings demonstrate that L. monocytogenes uses EVs for toxin release and implicate these structures in mammalian cytotoxicity.

Original languageEnglish (US)
Pages (from-to)1202-1217
Number of pages16
JournalJournal of Biological Chemistry
Volume294
Issue number4
DOIs
StatePublished - Jan 1 2019

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Listeria
Listeria monocytogenes
Virulence Factors
Bacteria
Cells
Phosphoinositide Phospholipase C
Gram-Positive Bacteria
Metabolites
Lipids
Proteins
Peptidoglycan
Macrophages
Poisons
Cytotoxicity
Bioactivity
Fungi
Cell Wall
Toxicity
Virulence
Fluorescence

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Listeria monocytogenes virulence factors, including listeriolysin O, are secreted in biologically active extracellular vesicles. / Coelho, Carolina; Brown, Lisa; Maryam, Maria; Vij, Raghav; Smith, Daniel F.Q.; Burnet, Meagan C.; Kyle, Jennifer E.; Heyman, Heino M.; Ramirez, Jasmine; Prados-Rosales, Rafael; Lauvau, Gregoire; Nakayasu, Ernesto S.; Brady, Nathan R.; Hamacher-Brady, Anne; Coppens, Isabelle; Casadevall, Arturo.

In: Journal of Biological Chemistry, Vol. 294, No. 4, 01.01.2019, p. 1202-1217.

Research output: Contribution to journalArticle

Coelho, C, Brown, L, Maryam, M, Vij, R, Smith, DFQ, Burnet, MC, Kyle, JE, Heyman, HM, Ramirez, J, Prados-Rosales, R, Lauvau, G, Nakayasu, ES, Brady, NR, Hamacher-Brady, A, Coppens, I & Casadevall, A 2019, 'Listeria monocytogenes virulence factors, including listeriolysin O, are secreted in biologically active extracellular vesicles', Journal of Biological Chemistry, vol. 294, no. 4, pp. 1202-1217. https://doi.org/10.1074/jbc.RA118.006472
Coelho, Carolina ; Brown, Lisa ; Maryam, Maria ; Vij, Raghav ; Smith, Daniel F.Q. ; Burnet, Meagan C. ; Kyle, Jennifer E. ; Heyman, Heino M. ; Ramirez, Jasmine ; Prados-Rosales, Rafael ; Lauvau, Gregoire ; Nakayasu, Ernesto S. ; Brady, Nathan R. ; Hamacher-Brady, Anne ; Coppens, Isabelle ; Casadevall, Arturo. / Listeria monocytogenes virulence factors, including listeriolysin O, are secreted in biologically active extracellular vesicles. In: Journal of Biological Chemistry. 2019 ; Vol. 294, No. 4. pp. 1202-1217.
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AU - Prados-Rosales, Rafael

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AU - Nakayasu, Ernesto S.

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