Listeria-infected myeloid dendritic cells produce IFN-β, priming T cell activation

Hanping Feng, Dong Zhang, Deborah Palliser, Pengcheng Zhu, Shenghe Cai, Ann Schlesinger, Laura Maliszewski, Judy Lieberman

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

The intracellular bacterium Listeria monocytogenes infects dendritic cells (DC) and other APCs and induces potent cell-mediated protective immunity. However, heat-killed bacteria fail to do so. This study explored whether DC differentially respond to live and killed Listeria and how this affects T cell activation. To control for bacterial number, a replication-deficient strain, Lmdd, defective in D-alanine biosynthesis, was used. We found that DC internalize both live and heat-killed Lmdd and similarly up-regulate the expression of costimulatory molecules, a necessary step for T cell activation. However, only live Lmdd-infected DC stimulate T cells to express the early activation marker CD69 and enhance T cell activation upon TCR engagement. Infection with live, but not heat-killed, Lmdd induces myeloid DC to secrete copious amounts of IFN-β, which requires bacterial cytosolic invasion. Exposure to high concentrations of IFN-β sensitizes naive T cells for Ag-dependent activation.

Original languageEnglish (US)
Pages (from-to)421-432
Number of pages12
JournalJournal of Immunology
Volume175
Issue number1
StatePublished - Jul 1 2005

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Feng, H., Zhang, D., Palliser, D., Zhu, P., Cai, S., Schlesinger, A., Maliszewski, L., & Lieberman, J. (2005). Listeria-infected myeloid dendritic cells produce IFN-β, priming T cell activation. Journal of Immunology, 175(1), 421-432.