Listeria-infected myeloid dendritic cells produce IFN-β, priming T cell activation

Hanping Feng, Dong Zhang, Deborah Palliser, Pengcheng Zhu, Shenghe Cai, Ann Schlesinger, Laura Maliszewski, Judy Lieberman

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

The intracellular bacterium Listeria monocytogenes infects dendritic cells (DC) and other APCs and induces potent cell-mediated protective immunity. However, heat-killed bacteria fail to do so. This study explored whether DC differentially respond to live and killed Listeria and how this affects T cell activation. To control for bacterial number, a replication-deficient strain, Lmdd, defective in D-alanine biosynthesis, was used. We found that DC internalize both live and heat-killed Lmdd and similarly up-regulate the expression of costimulatory molecules, a necessary step for T cell activation. However, only live Lmdd-infected DC stimulate T cells to express the early activation marker CD69 and enhance T cell activation upon TCR engagement. Infection with live, but not heat-killed, Lmdd induces myeloid DC to secrete copious amounts of IFN-β, which requires bacterial cytosolic invasion. Exposure to high concentrations of IFN-β sensitizes naive T cells for Ag-dependent activation.

Original languageEnglish (US)
Pages (from-to)421-432
Number of pages12
JournalJournal of Immunology
Volume175
Issue number1
StatePublished - Jul 1 2005
Externally publishedYes

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Listeria
Myeloid Cells
Dendritic Cells
T-Lymphocytes
Hot Temperature
Bacteria
Listeria monocytogenes
Cellular Immunity
Alanine
Up-Regulation
Infection

ASJC Scopus subject areas

  • Immunology

Cite this

Feng, H., Zhang, D., Palliser, D., Zhu, P., Cai, S., Schlesinger, A., ... Lieberman, J. (2005). Listeria-infected myeloid dendritic cells produce IFN-β, priming T cell activation. Journal of Immunology, 175(1), 421-432.

Listeria-infected myeloid dendritic cells produce IFN-β, priming T cell activation. / Feng, Hanping; Zhang, Dong; Palliser, Deborah; Zhu, Pengcheng; Cai, Shenghe; Schlesinger, Ann; Maliszewski, Laura; Lieberman, Judy.

In: Journal of Immunology, Vol. 175, No. 1, 01.07.2005, p. 421-432.

Research output: Contribution to journalArticle

Feng, H, Zhang, D, Palliser, D, Zhu, P, Cai, S, Schlesinger, A, Maliszewski, L & Lieberman, J 2005, 'Listeria-infected myeloid dendritic cells produce IFN-β, priming T cell activation', Journal of Immunology, vol. 175, no. 1, pp. 421-432.
Feng H, Zhang D, Palliser D, Zhu P, Cai S, Schlesinger A et al. Listeria-infected myeloid dendritic cells produce IFN-β, priming T cell activation. Journal of Immunology. 2005 Jul 1;175(1):421-432.
Feng, Hanping ; Zhang, Dong ; Palliser, Deborah ; Zhu, Pengcheng ; Cai, Shenghe ; Schlesinger, Ann ; Maliszewski, Laura ; Lieberman, Judy. / Listeria-infected myeloid dendritic cells produce IFN-β, priming T cell activation. In: Journal of Immunology. 2005 ; Vol. 175, No. 1. pp. 421-432.
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