Lipopolysaccharide Induces Synthesis of Mouse Colony-Stimulating Factor-1 in Vivo

Philip Roth, Anna Bartocci, E. Richard Stanley

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

CSF-1 is a hemopoietic growth factor that regulates the survival, proliferation, and differentiation of mononuclear phagocytes, cells that are critical in the inflammatory response. In the case of Gram-negative infection, LPS plays an important role by inducing several cell types to produce the proinflammatory cytokines, IL-1, IL-6, and TNF-α. In this study, we examined the effects of i.p. administration of LPS on CSF-1 expression in the mouse. Two- to sevenfold increases in the CSF-1 concentrations determined by RIA were evident within hours of LPS administration in serum, liver, kidney, lung, spleen, brain, intestine, and heart. While alterations in the CSF-1 receptor-mediated clearance of CSF-1 appeared not to account for the increased growth factor concentrations in LPS-treated animals, there was an early LPS-induced reduction of splenic [125I]CSF-1 uptake consistent with tissue-specific down-modulation of CSF-1 receptors. The results of Northern analysis revealed increased expression of a CSF-1 mRNA species in liver, lung, kidney, spleen, intestine, and heart following LPS treatment, demonstrating that increased synthesis was responsible for the increased tissue CSF-1 concentrations. The increased expression and synthesis of CSF-1 in response to LPS may be essential for mobilizing and activating mononuclear phagocytes in the inflammatory response.

Original languageEnglish (US)
Pages (from-to)3874-3880
Number of pages7
JournalJournal of Immunology
Volume158
Issue number8
StatePublished - Apr 15 1997

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Macrophage Colony-Stimulating Factor
Lipopolysaccharides
Macrophage Colony-Stimulating Factor Receptors
Phagocytes
Intestines
Intercellular Signaling Peptides and Proteins
Spleen
Kidney
Lung
Liver
Interleukin-1
Interleukin-6
Cytokines
Messenger RNA
Brain
Infection
Serum

ASJC Scopus subject areas

  • Immunology

Cite this

Roth, P., Bartocci, A., & Stanley, E. R. (1997). Lipopolysaccharide Induces Synthesis of Mouse Colony-Stimulating Factor-1 in Vivo. Journal of Immunology, 158(8), 3874-3880.

Lipopolysaccharide Induces Synthesis of Mouse Colony-Stimulating Factor-1 in Vivo. / Roth, Philip; Bartocci, Anna; Stanley, E. Richard.

In: Journal of Immunology, Vol. 158, No. 8, 15.04.1997, p. 3874-3880.

Research output: Contribution to journalArticle

Roth, P, Bartocci, A & Stanley, ER 1997, 'Lipopolysaccharide Induces Synthesis of Mouse Colony-Stimulating Factor-1 in Vivo', Journal of Immunology, vol. 158, no. 8, pp. 3874-3880.
Roth, Philip ; Bartocci, Anna ; Stanley, E. Richard. / Lipopolysaccharide Induces Synthesis of Mouse Colony-Stimulating Factor-1 in Vivo. In: Journal of Immunology. 1997 ; Vol. 158, No. 8. pp. 3874-3880.
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