Lipid length controls antigen entry into endosomal and nonendosomal pathways for CD1b presentation

D. Branch Moody, Volker Briken, Tan Yun Cheng, Carme Roura-Mir, Mark R. Guy, David H. Geho, Mark L. Tykocinski, Gurdyal S. Besra, Steven A. Porcelli

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

CDI proteins present various glycolipid antigens to T cells, but the cellular mechanisms that control which particular glycolipids generate T cell responses are not understood. We show here that T cell recognition of glucose monomycolate antigens with long (C80) alkyl chains involves the delivery of CD1b proteins and antigens to late endosomes in a process that takes several hours. In contrast, analogs of the same antigen with shorter (C32) alkyl chains are rapidly, but inefficiently, presented by cell surface CD1b proteins. Dendritic cells (DCs) preferentially present long-chain glycolipids, which results, in part, from their rapid internalization and selective delivery of antigens to endosomal compartments. Nonprofessional antigen-presenting cells, however, preferentially present short-chain glycolipids because of their lack of prominent endosomal presentation pathways. Because long alkyl chain length distinguishes certain microbial glycolipids from common mammalian glycolipids, these findings suggest that DCs use a specialized endosomal-loading pathway to promote preferential recognition of glycolipids with a more intrinsically foreign structure.

Original languageEnglish (US)
Pages (from-to)435-442
Number of pages8
JournalNature Immunology
Volume3
Issue number5
DOIs
StatePublished - 2002

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Glycolipids
Lipids
Antigens
T-Lymphocytes
Dendritic Cells
Endosomes
Antigen-Presenting Cells
Membrane Proteins
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Lipid length controls antigen entry into endosomal and nonendosomal pathways for CD1b presentation. / Moody, D. Branch; Briken, Volker; Cheng, Tan Yun; Roura-Mir, Carme; Guy, Mark R.; Geho, David H.; Tykocinski, Mark L.; Besra, Gurdyal S.; Porcelli, Steven A.

In: Nature Immunology, Vol. 3, No. 5, 2002, p. 435-442.

Research output: Contribution to journalArticle

Moody, DB, Briken, V, Cheng, TY, Roura-Mir, C, Guy, MR, Geho, DH, Tykocinski, ML, Besra, GS & Porcelli, SA 2002, 'Lipid length controls antigen entry into endosomal and nonendosomal pathways for CD1b presentation', Nature Immunology, vol. 3, no. 5, pp. 435-442. https://doi.org/10.1038/ni780
Moody, D. Branch ; Briken, Volker ; Cheng, Tan Yun ; Roura-Mir, Carme ; Guy, Mark R. ; Geho, David H. ; Tykocinski, Mark L. ; Besra, Gurdyal S. ; Porcelli, Steven A. / Lipid length controls antigen entry into endosomal and nonendosomal pathways for CD1b presentation. In: Nature Immunology. 2002 ; Vol. 3, No. 5. pp. 435-442.
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AU - Guy, Mark R.

AU - Geho, David H.

AU - Tykocinski, Mark L.

AU - Besra, Gurdyal S.

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