Lipid fluidity of hepatocyte plasma membrane subfractions and their differential regulation by calcium

Judith Storch; David Schachter; Masayasu Inoue; Allan W. Wolkoff

doi:10.1016/0005-2736(83)90386-3

Lipid fluidity of hepatocyte plasma membrane subfractions and their differential regulation by calcium

Judith Storch, David Schachter, Masayasu Inoue, Allan W. Wolkoff

Medicine

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Rat hepatocyte plasma membranes were subfractionated by several methods into canalicular, sinusoidal and mixed contiguous plus sinusoidal membranes. Assessment of lipid fluidity by steady-state fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene and 12-(9-anthroyloxy)stearate indicates that the canalicular fraction is less fluid than the other membranes. Incubation with calcium decreases the fluidity of the sinusoidal and contiguous membranes by altering the lipid composition, an action which is not reversed by subsequent chelation of the cation. This effect of calcium is not observed in canalicular membranes.

Original language	English (US)
Pages (from-to)	209-212
Number of pages	4
Journal	BBA - Biomembranes
Volume	727
Issue number	1
DOIs	https://doi.org/10.1016/0005-2736(83)90386-3
State	Published - Jan 5 1983

Keywords

(Rat hepatocyte)
Ca
Fluorescence polarization
Lipid composition
Lipid fluidity
Plasma membrane

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

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10.1016/0005-2736(83)90386-3

Cite this

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title = "Lipid fluidity of hepatocyte plasma membrane subfractions and their differential regulation by calcium",

abstract = "Rat hepatocyte plasma membranes were subfractionated by several methods into canalicular, sinusoidal and mixed contiguous plus sinusoidal membranes. Assessment of lipid fluidity by steady-state fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene and 12-(9-anthroyloxy)stearate indicates that the canalicular fraction is less fluid than the other membranes. Incubation with calcium decreases the fluidity of the sinusoidal and contiguous membranes by altering the lipid composition, an action which is not reversed by subsequent chelation of the cation. This effect of calcium is not observed in canalicular membranes.",

keywords = "(Rat hepatocyte), Ca, Fluorescence polarization, Lipid composition, Lipid fluidity, Plasma membrane",

author = "Judith Storch and David Schachter and Masayasu Inoue and Wolkoff, {Allan W.}",

note = "Funding Information: The support of National Institutes of Health grants AM-21238, AM-07330, AM-17702, AM-02019, GM-27859 and AM-23026 is gratefully acknowledged.",

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AU - Storch, Judith

AU - Schachter, David

AU - Inoue, Masayasu

AU - Wolkoff, Allan W.

N1 - Funding Information: The support of National Institutes of Health grants AM-21238, AM-07330, AM-17702, AM-02019, GM-27859 and AM-23026 is gratefully acknowledged.

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N2 - Rat hepatocyte plasma membranes were subfractionated by several methods into canalicular, sinusoidal and mixed contiguous plus sinusoidal membranes. Assessment of lipid fluidity by steady-state fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene and 12-(9-anthroyloxy)stearate indicates that the canalicular fraction is less fluid than the other membranes. Incubation with calcium decreases the fluidity of the sinusoidal and contiguous membranes by altering the lipid composition, an action which is not reversed by subsequent chelation of the cation. This effect of calcium is not observed in canalicular membranes.

AB - Rat hepatocyte plasma membranes were subfractionated by several methods into canalicular, sinusoidal and mixed contiguous plus sinusoidal membranes. Assessment of lipid fluidity by steady-state fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene and 12-(9-anthroyloxy)stearate indicates that the canalicular fraction is less fluid than the other membranes. Incubation with calcium decreases the fluidity of the sinusoidal and contiguous membranes by altering the lipid composition, an action which is not reversed by subsequent chelation of the cation. This effect of calcium is not observed in canalicular membranes.

KW - (Rat hepatocyte)

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Lipid fluidity of hepatocyte plasma membrane subfractions and their differential regulation by calcium

Abstract

Keywords

ASJC Scopus subject areas

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