Lipid fluidity of hepatocyte plasma membrane subfractions and their differential regulation by calcium

Judith Storch, David Schachter, Masayasu Inoue, Allan W. Wolkoff

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Rat hepatocyte plasma membranes were subfractionated by several methods into canalicular, sinusoidal and mixed contiguous plus sinusoidal membranes. Assessment of lipid fluidity by steady-state fluorescence polarization of 1,6-diphenyl-1,3,5-hexatriene and 12-(9-anthroyloxy)stearate indicates that the canalicular fraction is less fluid than the other membranes. Incubation with calcium decreases the fluidity of the sinusoidal and contiguous membranes by altering the lipid composition, an action which is not reversed by subsequent chelation of the cation. This effect of calcium is not observed in canalicular membranes.

Original languageEnglish (US)
Pages (from-to)209-212
Number of pages4
JournalBBA - Biomembranes
Volume727
Issue number1
DOIs
StatePublished - Jan 5 1983

Fingerprint

Fluidity
Cell membranes
Hepatocytes
Cell Membrane
Calcium
Membranes
Lipids
Diphenylhexatriene
Stearates
Fluorescence Polarization
Membrane Lipids
Cations
Chelation
Rats
Fluorescence
Polarization
Fluids
Chemical analysis

Keywords

  • (Rat hepatocyte)
  • Ca
  • Fluorescence polarization
  • Lipid composition
  • Lipid fluidity
  • Plasma membrane

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Medicine(all)

Cite this

Lipid fluidity of hepatocyte plasma membrane subfractions and their differential regulation by calcium. / Storch, Judith; Schachter, David; Inoue, Masayasu; Wolkoff, Allan W.

In: BBA - Biomembranes, Vol. 727, No. 1, 05.01.1983, p. 209-212.

Research output: Contribution to journalArticle

Storch, Judith ; Schachter, David ; Inoue, Masayasu ; Wolkoff, Allan W. / Lipid fluidity of hepatocyte plasma membrane subfractions and their differential regulation by calcium. In: BBA - Biomembranes. 1983 ; Vol. 727, No. 1. pp. 209-212.
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