Lipase 8 affects the pathogenesis of Candida albicans

Attila Gácser; Frank Stehr; Cathrin Kröger; László Kredics; Wilhelm Schäfer; Joshua D. Nosanchuk

doi:10.1128/IAI.00372-07

Lipase 8 affects the pathogenesis of Candida albicans

Attila Gácser, Frank Stehr, Cathrin Kröger, László Kredics, Wilhelm Schäfer, Joshua D. Nosanchuk

Medicine

Research output: Contribution to journal › Article › peer-review

74 Scopus citations

Abstract

The production of lipases can affect microbial fitness and virulence. We examined the role of the lipase 8 (LIP8) gene in the virulence of Candida albicans by constructing Δlip8 strains by the URA-blaster disruption method. Reverse transcription-PCR experiments demonstrated the absence of LIP8 expression in the homozygous knockout mutants. Reconstituted strains and overexpression mutants were generated by introducing a LIP8 open reading frame under control of a constitutive actin promoter. Knockout mutants produced more mycelium, particularly at higher temperatures and pH ≥7. Diminished LIP8 expression resulted in reduced growth in lipid-containing media. Mutants deficient in the LIP8 gene were significantly less virulent in a murine intravenous infection model. The results clearly indicate that Lip8p is an important virulence factor of C. albicans.

Original language	English (US)
Pages (from-to)	4710-4718
Number of pages	9
Journal	Infection and immunity
Volume	75
Issue number	10
DOIs	https://doi.org/10.1128/IAI.00372-07
State	Published - Oct 2007

ASJC Scopus subject areas

Parasitology
Microbiology
Immunology
Infectious Diseases

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1128/IAI.00372-07

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abstract = "The production of lipases can affect microbial fitness and virulence. We examined the role of the lipase 8 (LIP8) gene in the virulence of Candida albicans by constructing Δlip8 strains by the URA-blaster disruption method. Reverse transcription-PCR experiments demonstrated the absence of LIP8 expression in the homozygous knockout mutants. Reconstituted strains and overexpression mutants were generated by introducing a LIP8 open reading frame under control of a constitutive actin promoter. Knockout mutants produced more mycelium, particularly at higher temperatures and pH ≥7. Diminished LIP8 expression resulted in reduced growth in lipid-containing media. Mutants deficient in the LIP8 gene were significantly less virulent in a murine intravenous infection model. The results clearly indicate that Lip8p is an important virulence factor of C. albicans.",

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AU - Gácser, Attila

AU - Stehr, Frank

AU - Kröger, Cathrin

AU - Kredics, László

AU - Schäfer, Wilhelm

AU - Nosanchuk, Joshua D.

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N2 - The production of lipases can affect microbial fitness and virulence. We examined the role of the lipase 8 (LIP8) gene in the virulence of Candida albicans by constructing Δlip8 strains by the URA-blaster disruption method. Reverse transcription-PCR experiments demonstrated the absence of LIP8 expression in the homozygous knockout mutants. Reconstituted strains and overexpression mutants were generated by introducing a LIP8 open reading frame under control of a constitutive actin promoter. Knockout mutants produced more mycelium, particularly at higher temperatures and pH ≥7. Diminished LIP8 expression resulted in reduced growth in lipid-containing media. Mutants deficient in the LIP8 gene were significantly less virulent in a murine intravenous infection model. The results clearly indicate that Lip8p is an important virulence factor of C. albicans.

AB - The production of lipases can affect microbial fitness and virulence. We examined the role of the lipase 8 (LIP8) gene in the virulence of Candida albicans by constructing Δlip8 strains by the URA-blaster disruption method. Reverse transcription-PCR experiments demonstrated the absence of LIP8 expression in the homozygous knockout mutants. Reconstituted strains and overexpression mutants were generated by introducing a LIP8 open reading frame under control of a constitutive actin promoter. Knockout mutants produced more mycelium, particularly at higher temperatures and pH ≥7. Diminished LIP8 expression resulted in reduced growth in lipid-containing media. Mutants deficient in the LIP8 gene were significantly less virulent in a murine intravenous infection model. The results clearly indicate that Lip8p is an important virulence factor of C. albicans.

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Lipase 8 affects the pathogenesis of Candida albicans

Abstract

ASJC Scopus subject areas

UN SDGs

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