Lipase 8 affects the pathogenesis of Candida albicans

Attila Gácser, Frank Stehr, Cathrin Kröger, László Kredics, Wilhelm Schäfer, Joshua D. Nosanchuk

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The production of lipases can affect microbial fitness and virulence. We examined the role of the lipase 8 (LIP8) gene in the virulence of Candida albicans by constructing Δlip8 strains by the URA-blaster disruption method. Reverse transcription-PCR experiments demonstrated the absence of LIP8 expression in the homozygous knockout mutants. Reconstituted strains and overexpression mutants were generated by introducing a LIP8 open reading frame under control of a constitutive actin promoter. Knockout mutants produced more mycelium, particularly at higher temperatures and pH ≥7. Diminished LIP8 expression resulted in reduced growth in lipid-containing media. Mutants deficient in the LIP8 gene were significantly less virulent in a murine intravenous infection model. The results clearly indicate that Lip8p is an important virulence factor of C. albicans.

Original languageEnglish (US)
Pages (from-to)4710-4718
Number of pages9
JournalInfection and Immunity
Volume75
Issue number10
DOIs
StatePublished - Oct 2007

Fingerprint

Lipase
Candida albicans
Virulence
Mycelium
Virulence Factors
Open Reading Frames
Genes
Reverse Transcription
Actins
Lipids
Polymerase Chain Reaction
Temperature
Growth
Infection

ASJC Scopus subject areas

  • Immunology

Cite this

Gácser, A., Stehr, F., Kröger, C., Kredics, L., Schäfer, W., & Nosanchuk, J. D. (2007). Lipase 8 affects the pathogenesis of Candida albicans. Infection and Immunity, 75(10), 4710-4718. https://doi.org/10.1128/IAI.00372-07

Lipase 8 affects the pathogenesis of Candida albicans. / Gácser, Attila; Stehr, Frank; Kröger, Cathrin; Kredics, László; Schäfer, Wilhelm; Nosanchuk, Joshua D.

In: Infection and Immunity, Vol. 75, No. 10, 10.2007, p. 4710-4718.

Research output: Contribution to journalArticle

Gácser, A, Stehr, F, Kröger, C, Kredics, L, Schäfer, W & Nosanchuk, JD 2007, 'Lipase 8 affects the pathogenesis of Candida albicans', Infection and Immunity, vol. 75, no. 10, pp. 4710-4718. https://doi.org/10.1128/IAI.00372-07
Gácser A, Stehr F, Kröger C, Kredics L, Schäfer W, Nosanchuk JD. Lipase 8 affects the pathogenesis of Candida albicans. Infection and Immunity. 2007 Oct;75(10):4710-4718. https://doi.org/10.1128/IAI.00372-07
Gácser, Attila ; Stehr, Frank ; Kröger, Cathrin ; Kredics, László ; Schäfer, Wilhelm ; Nosanchuk, Joshua D. / Lipase 8 affects the pathogenesis of Candida albicans. In: Infection and Immunity. 2007 ; Vol. 75, No. 10. pp. 4710-4718.
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