Lipase 8 affects the pathogenesis of Candida albicans

Attila Gácser, Frank Stehr, Cathrin Kröger, László Kredics, Wilhelm Schäfer, Joshua D. Nosanchuk

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47 Scopus citations

Abstract

The production of lipases can affect microbial fitness and virulence. We examined the role of the lipase 8 (LIP8) gene in the virulence of Candida albicans by constructing Δlip8 strains by the URA-blaster disruption method. Reverse transcription-PCR experiments demonstrated the absence of LIP8 expression in the homozygous knockout mutants. Reconstituted strains and overexpression mutants were generated by introducing a LIP8 open reading frame under control of a constitutive actin promoter. Knockout mutants produced more mycelium, particularly at higher temperatures and pH ≥7. Diminished LIP8 expression resulted in reduced growth in lipid-containing media. Mutants deficient in the LIP8 gene were significantly less virulent in a murine intravenous infection model. The results clearly indicate that Lip8p is an important virulence factor of C. albicans.

Original languageEnglish (US)
Pages (from-to)4710-4718
Number of pages9
JournalInfection and immunity
Volume75
Issue number10
DOIs
StatePublished - Oct 1 2007

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ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Gácser, A., Stehr, F., Kröger, C., Kredics, L., Schäfer, W., & Nosanchuk, J. D. (2007). Lipase 8 affects the pathogenesis of Candida albicans. Infection and immunity, 75(10), 4710-4718. https://doi.org/10.1128/IAI.00372-07