Linkage analysis by two-dimensional DNA typing

Gerard J. Te Meerman, Erik Mullaart, Martin A. Van Der Meulen, Johanna H G Den Daas, Bruno Morolli, André G. Uitterlinden, Jan Vijg

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

In two-dimensional (2-D) DNA typing, genomic DNA fragments are separated, first according to size by electrophoresis in a neutral polyacrylamide gel and second according to sequence by denaturing gradient gel electrophoresis, followed by hybridization analysis using micro- and minisatellite core probes. The 2-D DNA typing method generates a large amount of information on polymorphic loci per gel. Here we demonstrate the potential usefulness of 2-D DNA typing in an empirical linkage study on the red factor in cattle, and we show an example of the 2-D DNA typing analysis of a human pedigree. The power efficiency of 2-D DNA typing in general is compared with that of single-locus typing by simulation. The results indicate that, although 2-D DNA typing is very efficient in generating data on polymorphic loci, its power to detect linkage is lower than single-locus typing, because it is not obvious whether a spot represents the presence of one or two alleles. It is possible to compensate for this lower informativeness by increasing the sample size. Genome scanning by 2-D DNA typing has the potential to be more efficient than current genotyping methods in scoring polymorphic loci. Hence, it could become a method of choice in mapping genetic traits in humans and animals.

Original languageEnglish (US)
Pages (from-to)1289-1297
Number of pages9
JournalAmerican Journal of Human Genetics
Volume53
Issue number6
StatePublished - Dec 1993
Externally publishedYes

Fingerprint

DNA Fingerprinting
Denaturing Gradient Gel Electrophoresis
Minisatellite Repeats
Pedigree
Microsatellite Repeats
Sample Size
Electrophoresis
Research Design
Gels
Alleles
Genome
DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Te Meerman, G. J., Mullaart, E., Van Der Meulen, M. A., Den Daas, J. H. G., Morolli, B., Uitterlinden, A. G., & Vijg, J. (1993). Linkage analysis by two-dimensional DNA typing. American Journal of Human Genetics, 53(6), 1289-1297.

Linkage analysis by two-dimensional DNA typing. / Te Meerman, Gerard J.; Mullaart, Erik; Van Der Meulen, Martin A.; Den Daas, Johanna H G; Morolli, Bruno; Uitterlinden, André G.; Vijg, Jan.

In: American Journal of Human Genetics, Vol. 53, No. 6, 12.1993, p. 1289-1297.

Research output: Contribution to journalArticle

Te Meerman, GJ, Mullaart, E, Van Der Meulen, MA, Den Daas, JHG, Morolli, B, Uitterlinden, AG & Vijg, J 1993, 'Linkage analysis by two-dimensional DNA typing', American Journal of Human Genetics, vol. 53, no. 6, pp. 1289-1297.
Te Meerman GJ, Mullaart E, Van Der Meulen MA, Den Daas JHG, Morolli B, Uitterlinden AG et al. Linkage analysis by two-dimensional DNA typing. American Journal of Human Genetics. 1993 Dec;53(6):1289-1297.
Te Meerman, Gerard J. ; Mullaart, Erik ; Van Der Meulen, Martin A. ; Den Daas, Johanna H G ; Morolli, Bruno ; Uitterlinden, André G. ; Vijg, Jan. / Linkage analysis by two-dimensional DNA typing. In: American Journal of Human Genetics. 1993 ; Vol. 53, No. 6. pp. 1289-1297.
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