Lineage specific receptors used to identify a growth factor for developmentally early hemopoietic cells: Assay of hemopoietin‐2

Stephen H. Bartelmez, Rosalba Sacca, E. Richard Stanley

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

A new approach, based on the occurrence of receptors for the mononuclear phagocyte lineage specific hemopoietic growth factor (HGF) colony stimulating ractor‐1 (CSF‐1) on developmentally early multipotent cells, is utilized to detect and assay rapidly another HGF, hemopoietin‐2. This method is also used to determine the relative maturity of hemopoietin‐2 target cells, to investigate synergism between hemopoietin‐2 and CSF‐1, and to measure CSF‐1 receptor levels on maturing cells. While the target cell specificities of hemopoitin‐2 and CSF‐1 overlap, hemopoietin‐2 causes the appearance of developmentally earlier 125I‐CSF‐1 binding cells de novo in the absence of CSF‐1. Increased CSF‐1 receptor densities are observed on cells incubated with either HGF, consistent with acquisition of the capacity for increased expression of the receptor by mononuclear phagocyte progenitor cells just prior to their differentiation to adherent mononuclear phagocytes. Together, both HGFs have a synergistic effect on the generation of 125I‐CSF‐1 binding cells with elevated CSF‐1 receptor densities. Preliminary characterization of hemopoietin‐2 from medium conditioned by WEHI‐3 cells indicates that it is very similar to, if not identical with, interleukin‐3 (IL‐3) and the HGF(s) acting on multipotential cells and cells giving rise to erythroid cells, granulocytes, mononuclear phagocytes, and megakaryocytes. Purified IL‐3 was shown to possess hemopoietin‐2 activity.

Original languageEnglish (US)
Pages (from-to)362-369
Number of pages8
JournalJournal of Cellular Physiology
Volume122
Issue number3
DOIs
StatePublished - Mar 1985

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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