Limited variation of the 5′cis-control region of the transmission blocking vaccine candidate Pfs25 amid great genetic diversity of Plasmodium falciparum in Cameroon

Wilfred Fon Mbacham, Patrice Nsangou Mimche, Palmer Masumbe Netongo, Evehe Bebandoue Marie-Solange, Akindeh Nji, Immaculate Amunom, Johanna P. Daily, Valerie Makoge, Kayla Laserson, Songmbe Michael Yong, Nicoline Lomah, Peter Enyong, Vincent P. Titanji, Dyann F. Wirth

Research output: Contribution to journalArticle

Abstract

Genetic recombination during sexual reproduction within Plasmodium sp. contributes to parasite diversity and altered gene expression of certain surface markers. The pfs25 gene involved in the upset of gametocytogenesis is a candidate antigen in transmission blocking vaccine. This study investigated the polymorphism of Pfs25 within its 5′cis-control region in field isolates from different ecotypes in Cameroon. Symptomatic patients and asymptomatic healthy school children with a positive smear and from different ecozones were included. Parasite DNA was extracted and polymorphisms within pfs25, cg2-ω, msp-1, msp-2 and glurp genes were investigated by PCR-RFLP and DNA sequencing. Putative control elements of the 5′cis control regions of Pfs25 were identified by PCGENE software and enzymes were selected whose sequences produced or abolished restriction sites by mutations. Malaria infection was mainly caused by Plasmodium falciparum with sporadic occurrence of Plasmodium malariae and Plasmodium ovale. Analysis of the Pfs25 5′ cis-control region identified only one polymorphism (0.002%) that abolished an RsaI restriction site as part of the sequence TTTCTGTAC, located 40 bp downstream of the promoter and found at - 478 bp of the ATG. Analysis of the 5′ cis-control sequence of Pfs25 revealed minimal variation of the promoter region amid great zonal differences in parasite population. Altitudinal differences in parasite populations were not easily discernable.

Original languageEnglish (US)
Pages (from-to)523-533
Number of pages11
JournalAfrican Journal of Biotechnology
Volume7
Issue number5
StatePublished - Mar 4 2008
Externally publishedYes

Fingerprint

Cameroon
Plasmodium falciparum
Parasites
Vaccines
vaccines
parasites
genetic variation
genetic polymorphism
Plasmodium ovale
Plasmodium malariae
promoter regions
Ecotype
genetic recombination
ecological zones
Plasmodium
school children
sexual reproduction
ecotypes
DNA Sequence Analysis
Genetic Promoter Regions

Keywords

  • Cis-control elements
  • Genetic polymorphism
  • Pfs25
  • Plasmodium falciparum

ASJC Scopus subject areas

  • Biotechnology

Cite this

Mbacham, W. F., Mimche, P. N., Netongo, P. M., Marie-Solange, E. B., Nji, A., Amunom, I., ... Wirth, D. F. (2008). Limited variation of the 5′cis-control region of the transmission blocking vaccine candidate Pfs25 amid great genetic diversity of Plasmodium falciparum in Cameroon. African Journal of Biotechnology, 7(5), 523-533.

Limited variation of the 5′cis-control region of the transmission blocking vaccine candidate Pfs25 amid great genetic diversity of Plasmodium falciparum in Cameroon. / Mbacham, Wilfred Fon; Mimche, Patrice Nsangou; Netongo, Palmer Masumbe; Marie-Solange, Evehe Bebandoue; Nji, Akindeh; Amunom, Immaculate; Daily, Johanna P.; Makoge, Valerie; Laserson, Kayla; Yong, Songmbe Michael; Lomah, Nicoline; Enyong, Peter; Titanji, Vincent P.; Wirth, Dyann F.

In: African Journal of Biotechnology, Vol. 7, No. 5, 04.03.2008, p. 523-533.

Research output: Contribution to journalArticle

Mbacham, WF, Mimche, PN, Netongo, PM, Marie-Solange, EB, Nji, A, Amunom, I, Daily, JP, Makoge, V, Laserson, K, Yong, SM, Lomah, N, Enyong, P, Titanji, VP & Wirth, DF 2008, 'Limited variation of the 5′cis-control region of the transmission blocking vaccine candidate Pfs25 amid great genetic diversity of Plasmodium falciparum in Cameroon', African Journal of Biotechnology, vol. 7, no. 5, pp. 523-533.
Mbacham, Wilfred Fon ; Mimche, Patrice Nsangou ; Netongo, Palmer Masumbe ; Marie-Solange, Evehe Bebandoue ; Nji, Akindeh ; Amunom, Immaculate ; Daily, Johanna P. ; Makoge, Valerie ; Laserson, Kayla ; Yong, Songmbe Michael ; Lomah, Nicoline ; Enyong, Peter ; Titanji, Vincent P. ; Wirth, Dyann F. / Limited variation of the 5′cis-control region of the transmission blocking vaccine candidate Pfs25 amid great genetic diversity of Plasmodium falciparum in Cameroon. In: African Journal of Biotechnology. 2008 ; Vol. 7, No. 5. pp. 523-533.
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