Limited nuclease digestion of heterogeneous nuclear ribonucleoprotein in nuclei

Miryam Z. Wahrman, Leonard H. Augenlicht

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

We have used limited nuclease digestion of nuclei to probe the structure of nuclear ribonucleoprotein (nRNP). Analysis of [3H]uridine-labeled heterogeneous nuclear RNA isolated from nuclease digested nuclei revealed preferential generation of discrete bands of RNA ranging in size from 1.5 × 105 to 6 × 105 daltons. The nuclease digestion pattern of nRNP differed from the nuclease digestion pattern obtained with chromatin in that the RNA bands generated in these experiments were transient, appearing only early in the course of digestion, and no stable nRNP monomer size was evident. Therefore, although nRNP may be organized in a regular configuration, nRNP structure differs considerably from the repeating subunit structure of chromatin.

Original languageEnglish (US)
Pages (from-to)395-402
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume87
Issue number2
DOIs
StatePublished - Mar 30 1979
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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