Limitations of CA125 as an index of peritoneal mesothelial cell mass: An in vitro study

Andrzej Brȩborowicz, Maciej Brȩborowicz, Malgorzata Pyda, Alicja Połubinska, Dimitrios Oreopoulos

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Background: CA125 is commonly used as an index of the mesothelial cell mass in patients treated with peritoneal dialysis. However, we have no data that show a direct relationship between the number of mesothelial cells, their functional properties, and the amount of CA125 produced in these cells. Methods: Experiments were performed on primary in vitro cultures of human peritoneal mesothelial cells obtained from 32 donors of various ages and of both sexes. Spontaneous release of CA125 from the confluent mesothelial cells was measured and correlated with the number of cells in monolayers and with their functional properties. We also studied acute effects of cytokines (IL-1β, TNF-α, and INF-γ) and the chronic effects of glucose (45 mM) on the CA125 content in mesothelial cells and the release of this antigen from their cytosol. Results: Cells from older donors released more CA125, but we found no correlation between the number of cells and the amount of CA125 released from their cytosol. The synthesis of CA125 in mesothelial cells does not correlate with the amount of monocyte chemoattractant protein 1 or interleukin-6 produced in these cells. Acute exposure to cytokines did not modify CA125 content or its release from mesothelial cells. Chronic exposure of mesothelial cells for 4 weeks to glucose (45 mM) decreased the CA125 content of their cytosol and the release of this antigen into the culture medium. Mannitol, at the same concentration and under the same conditions, did not produce these effects, namely a decrease in the CA125 content in the cytosol or its release into the cultural medium. Conclusions: The amount of CA125 released from mesothelial cells is not a good index of their number or their functional properties, because the CA125 release depends not only on the number of cells, but also on their properties. Furthermore, the process is affected by the age of the cell donor and environmental factors such as a high glucose content. The results of this study show the limitations of CA125 as an index of the mesothelial cell mass and viability.

Original languageEnglish (US)
JournalNephron - Clinical Practice
Volume100
Issue number2
DOIs
StatePublished - Jun 2005
Externally publishedYes

Fingerprint

Cytosol
Cell Count
Glucose
In Vitro Techniques
Tissue Donors
Cytokines
Antigens
Chemokine CCL2
Peritoneal Dialysis
Mannitol
Interleukin-1
Culture Media
Interleukin-6
Cell Survival

Keywords

  • CA125
  • Cytokines
  • Glucose
  • Mesothelial cell mass

ASJC Scopus subject areas

  • Nephrology

Cite this

Brȩborowicz, A., Brȩborowicz, M., Pyda, M., Połubinska, A., & Oreopoulos, D. (2005). Limitations of CA125 as an index of peritoneal mesothelial cell mass: An in vitro study. Nephron - Clinical Practice, 100(2). https://doi.org/10.1159/000085032

Limitations of CA125 as an index of peritoneal mesothelial cell mass : An in vitro study. / Brȩborowicz, Andrzej; Brȩborowicz, Maciej; Pyda, Malgorzata; Połubinska, Alicja; Oreopoulos, Dimitrios.

In: Nephron - Clinical Practice, Vol. 100, No. 2, 06.2005.

Research output: Contribution to journalArticle

Brȩborowicz, A, Brȩborowicz, M, Pyda, M, Połubinska, A & Oreopoulos, D 2005, 'Limitations of CA125 as an index of peritoneal mesothelial cell mass: An in vitro study', Nephron - Clinical Practice, vol. 100, no. 2. https://doi.org/10.1159/000085032
Brȩborowicz, Andrzej ; Brȩborowicz, Maciej ; Pyda, Malgorzata ; Połubinska, Alicja ; Oreopoulos, Dimitrios. / Limitations of CA125 as an index of peritoneal mesothelial cell mass : An in vitro study. In: Nephron - Clinical Practice. 2005 ; Vol. 100, No. 2.
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AU - Połubinska, Alicja

AU - Oreopoulos, Dimitrios

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