Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex

Christophe Rachez, Bryan D. Lemon, Zalman Suldan, Virginia Bromleigh, Matthew J. Gamble, Anders M. Näär, Hediye Erdjument-Bromage, Paul Tempst, Leonard P. Freedman

Research output: Contribution to journalArticle

606 Citations (Scopus)

Abstract

Nuclear receptors modulate the transcription of genes in direct response to small lipophilic ligands. Binding to ligands induces conformational changes in the nuclear receptors that enable the receptors to interact with several types of cofactor that are critical for transcription activation (transactivation). We previously described a distinct set of ligand-dependent proteins called DRIPs, which interact with the vitamin D receptor (VDR); together, these proteins constitute a new cofactor complex. DRIPs bind to several nuclear receptors and mediate ligand-dependent enhancement of transcription by VDR and the thyroid-hormone receptor in cell-free transcription assays. Here we report the identities of thirteen DRIPs that constitute this complex, and show that the complex has a central function in hormone-dependent transactivation by VDR on chromatin templates. The DRIPs are almost indistinguishable from components of another new cofactor complex called ARC, which is recruited by other types of transcription activators to mediate transactivation on chromatin-assembled templates. Several DRIP/ARC subunits are also components of other potentially related cofactors, such as CRSP, NAT, SMCC and the mouse Mediator, indicating that unique classes of activators may share common sets or subsets of cofactors. The role of nuclear-receptor ligands may in part, be to recruit such a cofactor complex to the receptor and, in doing so, to enhance transcription of target genes.

Original languageEnglish (US)
Pages (from-to)824-828
Number of pages5
JournalNature
Volume398
Issue number6730
DOIs
StatePublished - Apr 29 1999

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Cytoplasmic and Nuclear Receptors
Transcriptional Activation
Calcitriol Receptors
Ligands
Chromatin
AIDS-Related Complex
Thyroid Hormone Receptors
Genes
Proteins
VDR interacting protein complex DRIP
Hormones

ASJC Scopus subject areas

  • General

Cite this

Rachez, C., Lemon, B. D., Suldan, Z., Bromleigh, V., Gamble, M. J., Näär, A. M., ... Freedman, L. P. (1999). Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex. Nature, 398(6730), 824-828. https://doi.org/10.1038/19783

Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex. / Rachez, Christophe; Lemon, Bryan D.; Suldan, Zalman; Bromleigh, Virginia; Gamble, Matthew J.; Näär, Anders M.; Erdjument-Bromage, Hediye; Tempst, Paul; Freedman, Leonard P.

In: Nature, Vol. 398, No. 6730, 29.04.1999, p. 824-828.

Research output: Contribution to journalArticle

Rachez, C, Lemon, BD, Suldan, Z, Bromleigh, V, Gamble, MJ, Näär, AM, Erdjument-Bromage, H, Tempst, P & Freedman, LP 1999, 'Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex', Nature, vol. 398, no. 6730, pp. 824-828. https://doi.org/10.1038/19783
Rachez C, Lemon BD, Suldan Z, Bromleigh V, Gamble MJ, Näär AM et al. Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex. Nature. 1999 Apr 29;398(6730):824-828. https://doi.org/10.1038/19783
Rachez, Christophe ; Lemon, Bryan D. ; Suldan, Zalman ; Bromleigh, Virginia ; Gamble, Matthew J. ; Näär, Anders M. ; Erdjument-Bromage, Hediye ; Tempst, Paul ; Freedman, Leonard P. / Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex. In: Nature. 1999 ; Vol. 398, No. 6730. pp. 824-828.
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