Leucocyte behaviour in controlled ischaemia of the calves

G. Ciuffetti, M. Mercuri, Rita Lombardini, G. Maragoni, Laura Santambrogio, E. Mannarino

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Whole blood filterability and leucocyte behaviour (number, activation, and subfraction filterability rates) were monitored at the earliest stage ofperipheral ischaemia in 18 patients with stage II peripheral occlusive arterial disease (PAOD) and 20 matched controls. A model of controlled ischaemia, using exercise to stress leg circulation, was set up and blood samples were taken before exercise, at the onset of calf pain, and at recovery from peak exercise. Leucocytes were counted, separated into their subfractions on a Ficoll-Hypaque density gradient and by adhesion to Petri dishes, and filtered in buffer (like the whole blood suspensions) through 5 μm pore diameter Nuclepore filters. Unfractionated white cells, separated under gravity, with pseudopodia or cytoplasmic irregularities were regarded as activated. The whole blood filterability rate was significantly increased at the onset of calf pain and was associated with significant increases in the number of leucocytes and in the filterability rate of the monocyte sutifraction, the latter persisting throughout the recovery period. No significant changes were observed in the other variables monitored, showing that impairments in white cell rheology may be associated with ischaemia.

Original languageEnglish (US)
Pages (from-to)1083-1087
Number of pages5
JournalJournal of Clinical Pathology
Volume42
Issue number10
DOIs
StatePublished - 1989
Externally publishedYes

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Leukocytes
Ischemia
Exercise
Leukocyte Count
Diatrizoate
Pain
Ficoll
Pseudopodia
Rheology
Peripheral Arterial Disease
Gravitation
Monocytes
Leg
Suspensions
Buffers

ASJC Scopus subject areas

  • Health Policy
  • Pathology and Forensic Medicine

Cite this

Ciuffetti, G., Mercuri, M., Lombardini, R., Maragoni, G., Santambrogio, L., & Mannarino, E. (1989). Leucocyte behaviour in controlled ischaemia of the calves. Journal of Clinical Pathology, 42(10), 1083-1087. https://doi.org/10.1136/jcp.42.10.1083

Leucocyte behaviour in controlled ischaemia of the calves. / Ciuffetti, G.; Mercuri, M.; Lombardini, Rita; Maragoni, G.; Santambrogio, Laura; Mannarino, E.

In: Journal of Clinical Pathology, Vol. 42, No. 10, 1989, p. 1083-1087.

Research output: Contribution to journalArticle

Ciuffetti, G, Mercuri, M, Lombardini, R, Maragoni, G, Santambrogio, L & Mannarino, E 1989, 'Leucocyte behaviour in controlled ischaemia of the calves', Journal of Clinical Pathology, vol. 42, no. 10, pp. 1083-1087. https://doi.org/10.1136/jcp.42.10.1083
Ciuffetti G, Mercuri M, Lombardini R, Maragoni G, Santambrogio L, Mannarino E. Leucocyte behaviour in controlled ischaemia of the calves. Journal of Clinical Pathology. 1989;42(10):1083-1087. https://doi.org/10.1136/jcp.42.10.1083
Ciuffetti, G. ; Mercuri, M. ; Lombardini, Rita ; Maragoni, G. ; Santambrogio, Laura ; Mannarino, E. / Leucocyte behaviour in controlled ischaemia of the calves. In: Journal of Clinical Pathology. 1989 ; Vol. 42, No. 10. pp. 1083-1087.
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