Leptospires in the whistling frog (Eleutherodactylus johnstonei) on Barbados

C. O R Everard, D. G. Carrington, H. Korver, R. Burke, J. D. Everard, Claudia Gravekamp

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Two groups of whistling frogs (Eleutherodactylus johnstonei) comprising 99 and 117 animals were examined for leptospiral infection. Group I animals were caught in 14 areas of Barbados, and Group II animals in seven areas of suburban Bridgetown. Leptospires were isolated from the kidneys or body fluid of six frogs in Group I and the kidneys of 3 frogs in Group II. Two of the Group I isolates died out; the others were identified as bajan (a new serovar in the Australis serogroup) (6) and bim (Autumnalis) (1). The macerated body tissues and fluid of Group I frogs were put into phosphate buffered saline and examined by the microscopic agglutination test using 22 antigens. The results were all negative. For the Group II frogs the methodology was altered; blood was collected onto filter paper discs and allowed to dry out before being agitated in PBS and examined by the MAT. 15/117 (12.8%) animals were positive at ≥1:100 and 19 (16.2%) at ≥1:50. The geometric mean titre was 179. Seventeen of the sera reacted predominantly to antigens in the Australis serogroup, and two to Pyrogenes on its own. The serological results reflected the identity of the isolates. Serovars of Australis are not known to cause illness on Barbados, but bim is the commonest cause of severe leptospirosis on the island.

Original languageEnglish (US)
Pages (from-to)140-145
Number of pages6
JournalJournal of Tropical Medicine and Hygiene
Volume93
Issue number2
StatePublished - 1990
Externally publishedYes

Fingerprint

Barbados
Singing
frog
Anura
Animals
Antigens
animal
Body Fluids
antigen
Body fluids
Kidney
body fluid
Agglutination Tests
Leptospirosis
Phosphates
Blood
Islands
Tissue
serum
blood

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Everard, C. O. R., Carrington, D. G., Korver, H., Burke, R., Everard, J. D., & Gravekamp, C. (1990). Leptospires in the whistling frog (Eleutherodactylus johnstonei) on Barbados. Journal of Tropical Medicine and Hygiene, 93(2), 140-145.

Leptospires in the whistling frog (Eleutherodactylus johnstonei) on Barbados. / Everard, C. O R; Carrington, D. G.; Korver, H.; Burke, R.; Everard, J. D.; Gravekamp, Claudia.

In: Journal of Tropical Medicine and Hygiene, Vol. 93, No. 2, 1990, p. 140-145.

Research output: Contribution to journalArticle

Everard, COR, Carrington, DG, Korver, H, Burke, R, Everard, JD & Gravekamp, C 1990, 'Leptospires in the whistling frog (Eleutherodactylus johnstonei) on Barbados', Journal of Tropical Medicine and Hygiene, vol. 93, no. 2, pp. 140-145.
Everard, C. O R ; Carrington, D. G. ; Korver, H. ; Burke, R. ; Everard, J. D. ; Gravekamp, Claudia. / Leptospires in the whistling frog (Eleutherodactylus johnstonei) on Barbados. In: Journal of Tropical Medicine and Hygiene. 1990 ; Vol. 93, No. 2. pp. 140-145.
@article{c3172f808ab045aeb26a7fc1747a1282,
title = "Leptospires in the whistling frog (Eleutherodactylus johnstonei) on Barbados",
abstract = "Two groups of whistling frogs (Eleutherodactylus johnstonei) comprising 99 and 117 animals were examined for leptospiral infection. Group I animals were caught in 14 areas of Barbados, and Group II animals in seven areas of suburban Bridgetown. Leptospires were isolated from the kidneys or body fluid of six frogs in Group I and the kidneys of 3 frogs in Group II. Two of the Group I isolates died out; the others were identified as bajan (a new serovar in the Australis serogroup) (6) and bim (Autumnalis) (1). The macerated body tissues and fluid of Group I frogs were put into phosphate buffered saline and examined by the microscopic agglutination test using 22 antigens. The results were all negative. For the Group II frogs the methodology was altered; blood was collected onto filter paper discs and allowed to dry out before being agitated in PBS and examined by the MAT. 15/117 (12.8{\%}) animals were positive at ≥1:100 and 19 (16.2{\%}) at ≥1:50. The geometric mean titre was 179. Seventeen of the sera reacted predominantly to antigens in the Australis serogroup, and two to Pyrogenes on its own. The serological results reflected the identity of the isolates. Serovars of Australis are not known to cause illness on Barbados, but bim is the commonest cause of severe leptospirosis on the island.",
author = "Everard, {C. O R} and Carrington, {D. G.} and H. Korver and R. Burke and Everard, {J. D.} and Claudia Gravekamp",
year = "1990",
language = "English (US)",
volume = "93",
pages = "140--145",
journal = "Journal of Tropical Medicine and Hygiene",
issn = "0022-5304",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Leptospires in the whistling frog (Eleutherodactylus johnstonei) on Barbados

AU - Everard, C. O R

AU - Carrington, D. G.

AU - Korver, H.

AU - Burke, R.

AU - Everard, J. D.

AU - Gravekamp, Claudia

PY - 1990

Y1 - 1990

N2 - Two groups of whistling frogs (Eleutherodactylus johnstonei) comprising 99 and 117 animals were examined for leptospiral infection. Group I animals were caught in 14 areas of Barbados, and Group II animals in seven areas of suburban Bridgetown. Leptospires were isolated from the kidneys or body fluid of six frogs in Group I and the kidneys of 3 frogs in Group II. Two of the Group I isolates died out; the others were identified as bajan (a new serovar in the Australis serogroup) (6) and bim (Autumnalis) (1). The macerated body tissues and fluid of Group I frogs were put into phosphate buffered saline and examined by the microscopic agglutination test using 22 antigens. The results were all negative. For the Group II frogs the methodology was altered; blood was collected onto filter paper discs and allowed to dry out before being agitated in PBS and examined by the MAT. 15/117 (12.8%) animals were positive at ≥1:100 and 19 (16.2%) at ≥1:50. The geometric mean titre was 179. Seventeen of the sera reacted predominantly to antigens in the Australis serogroup, and two to Pyrogenes on its own. The serological results reflected the identity of the isolates. Serovars of Australis are not known to cause illness on Barbados, but bim is the commonest cause of severe leptospirosis on the island.

AB - Two groups of whistling frogs (Eleutherodactylus johnstonei) comprising 99 and 117 animals were examined for leptospiral infection. Group I animals were caught in 14 areas of Barbados, and Group II animals in seven areas of suburban Bridgetown. Leptospires were isolated from the kidneys or body fluid of six frogs in Group I and the kidneys of 3 frogs in Group II. Two of the Group I isolates died out; the others were identified as bajan (a new serovar in the Australis serogroup) (6) and bim (Autumnalis) (1). The macerated body tissues and fluid of Group I frogs were put into phosphate buffered saline and examined by the microscopic agglutination test using 22 antigens. The results were all negative. For the Group II frogs the methodology was altered; blood was collected onto filter paper discs and allowed to dry out before being agitated in PBS and examined by the MAT. 15/117 (12.8%) animals were positive at ≥1:100 and 19 (16.2%) at ≥1:50. The geometric mean titre was 179. Seventeen of the sera reacted predominantly to antigens in the Australis serogroup, and two to Pyrogenes on its own. The serological results reflected the identity of the isolates. Serovars of Australis are not known to cause illness on Barbados, but bim is the commonest cause of severe leptospirosis on the island.

UR - http://www.scopus.com/inward/record.url?scp=0025215822&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025215822&partnerID=8YFLogxK

M3 - Article

C2 - 2325195

AN - SCOPUS:0025215822

VL - 93

SP - 140

EP - 145

JO - Journal of Tropical Medicine and Hygiene

JF - Journal of Tropical Medicine and Hygiene

SN - 0022-5304

IS - 2

ER -