Leptin-sensitive sensory nerves innervate white fat.

Keegan T. Murphy, Gary J. Schwartz, Ngoc Ly T Nguyen, Jennifer M. Mendez, Vitaly Ryu, Timothy J. Bartness

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Leptin, the primary white adipose tissue (WAT) adipokine, is thought to convey lipid reserve information to the brain via the circulation. Because WAT responds to environmental/internal signals in a fat pad-specific (FPS) manner, systemic signals such as leptin would fail to communicate such distinctive information. Saturation of brain leptin transport systems also would fail to convey increased lipid levels beyond that point. WAT possesses sensory innervation exemplified by proven sensory-associated peptides in nerves within the tissue and by viral sensory nerve-specific transneuronal tract tracer, H129 strain of herpes simplex virus 1 labeling of dorsal root ganglia (DRG) pseudounipolar neurons, spinal cord and central sensory circuits. Leptin as a paracrine factor activating WAT sensory innervation could supply the brain with FPS information. Therefore, we tested for and found the presence of the long form of the leptin receptor (Ob-Rb) on DRG pseudounipolar neurons immunohistochemically labeled after injections of Fluorogold, a retrograde tract tracer, into inguinal WAT (IWAT). Intra-IWAT leptin injections (300 ng) significantly elevated IWAT nerve spike rate within 5 min and persisted for at least 30 min. Intra-IWAT leptin injections also induced significant c-Fos immunoreactivity (ir), indicating neural activation across DRG pseudounipolar sensory neurons labeled with Fluorogold IWAT injections. Intraperitoneal leptin injection did not increase c-Fos-ir in DRG or the arcuate nucleus, nor did it increase arcuate signal transducer and activator of transcription 3 phosphorylation-ir. Collectively, these results strongly suggest that endogenous leptin secreted from white adipocytes functions as a paracrine factor to activate spinal sensory nerves innervating the tissue.

Original languageEnglish (US)
JournalAmerican journal of physiology. Endocrinology and metabolism
Volume304
Issue number12
StatePublished - Jun 15 2013
Externally publishedYes

Fingerprint

White Adipose Tissue
Leptin
Groin
Spinal Ganglia
Nerve Tissue
Injections
Adipose Tissue
Brain
White Adipocytes
Lipids
Neurons
Leptin Receptors
STAT3 Transcription Factor
Spinal Nerves
Arcuate Nucleus of Hypothalamus
Adipokines
Human Herpesvirus 1
Sensory Receptor Cells
Intraperitoneal Injections
Spinal Cord

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Murphy, K. T., Schwartz, G. J., Nguyen, N. L. T., Mendez, J. M., Ryu, V., & Bartness, T. J. (2013). Leptin-sensitive sensory nerves innervate white fat. American journal of physiology. Endocrinology and metabolism, 304(12).

Leptin-sensitive sensory nerves innervate white fat. / Murphy, Keegan T.; Schwartz, Gary J.; Nguyen, Ngoc Ly T; Mendez, Jennifer M.; Ryu, Vitaly; Bartness, Timothy J.

In: American journal of physiology. Endocrinology and metabolism, Vol. 304, No. 12, 15.06.2013.

Research output: Contribution to journalArticle

Murphy, KT, Schwartz, GJ, Nguyen, NLT, Mendez, JM, Ryu, V & Bartness, TJ 2013, 'Leptin-sensitive sensory nerves innervate white fat.', American journal of physiology. Endocrinology and metabolism, vol. 304, no. 12.
Murphy, Keegan T. ; Schwartz, Gary J. ; Nguyen, Ngoc Ly T ; Mendez, Jennifer M. ; Ryu, Vitaly ; Bartness, Timothy J. / Leptin-sensitive sensory nerves innervate white fat. In: American journal of physiology. Endocrinology and metabolism. 2013 ; Vol. 304, No. 12.
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