Lens-specific expression of a chicken ßA3/A1-crystallin promoter fragment in transgenic mice

Joan B. McDermott, Ales Cvekl, Joram Piatigorsky

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

ßA3/A1-crystallin is one of the major refractive proteins of the chicken eye lens. Previously we showed that a fragment from -382 to +22 bp of the ßA3/A1-crystallin gene functions as a promoter in transfected lens cells. Here we show by use of the bacterial chloramphenicol acetyltransferase reporter gene that the -143/+22 fragment is sufficient for lens-specific promoter activity in transgenic mice. DNase I footprinting shows that lens nuclear proteins protect several regions within the minimal promoter fragment.

Original languageEnglish (US)
Pages (from-to)559-564
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume221
Issue number3
DOIs
Publication statusPublished - Apr 25 1996
Externally publishedYes

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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