Abstract
ßA3/A1-crystallin is one of the major refractive proteins of the chicken eye lens. Previously we showed that a fragment from -382 to +22 bp of the ßA3/A1-crystallin gene functions as a promoter in transfected lens cells. Here we show by use of the bacterial chloramphenicol acetyltransferase reporter gene that the -143/+22 fragment is sufficient for lens-specific promoter activity in transgenic mice. DNase I footprinting shows that lens nuclear proteins protect several regions within the minimal promoter fragment.
Original language | English (US) |
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Pages (from-to) | 559-564 |
Number of pages | 6 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 221 |
Issue number | 3 |
DOIs | |
State | Published - Apr 25 1996 |
Externally published | Yes |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology