Lectin-Resistant CHO Glycosylation Mutants

Santosh Kumar Patnaik, Pamela Stanley

Research output: Chapter in Book/Report/Conference proceedingChapter

134 Scopus citations

Abstract

Chinese hamster ovary (CHO) mutant cells with a wide variety of alterations in the glycosylation of proteins and lipids have been isolated by selection for resistance to the cytotoxicity of plant lectins. These CHO mutants have been used to characterize glycosylation pathways, to identify genes that code for glycosylation activities, to elucidate functional roles of glycans that mediate biological processes, and for glycosylation engineering. In this chapter, we briefly describe the available panel of lectin-resistant CHO mutants and summarize their glycan alterations and the biochemical and genetic bases of mutation.

Original languageEnglish (US)
Title of host publicationGlycomics
EditorsMinoru Fukuda
Pages159-182
Number of pages24
DOIs
StatePublished - Nov 20 2006
Externally publishedYes

Publication series

NameMethods in Enzymology
Volume416
ISSN (Print)0076-6879

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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  • Cite this

    Patnaik, S. K., & Stanley, P. (2006). Lectin-Resistant CHO Glycosylation Mutants. In M. Fukuda (Ed.), Glycomics (pp. 159-182). (Methods in Enzymology; Vol. 416). https://doi.org/10.1016/S0076-6879(06)16011-5