Abstract
Chinese hamster ovary (CHO) mutant cells with a wide variety of alterations in the glycosylation of proteins and lipids have been isolated by selection for resistance to the cytotoxicity of plant lectins. These CHO mutants have been used to characterize glycosylation pathways, to identify genes that code for glycosylation activities, to elucidate functional roles of glycans that mediate biological processes, and for glycosylation engineering. In this chapter, we briefly describe the available panel of lectin-resistant CHO mutants and summarize their glycan alterations and the biochemical and genetic bases of mutation.
| Original language | English (US) |
|---|---|
| Title of host publication | Glycomics |
| Editors | Minoru Fukuda |
| Pages | 159-182 |
| Number of pages | 24 |
| DOIs | |
| State | Published - Nov 20 2006 |
| Externally published | Yes |
Publication series
| Name | Methods in Enzymology |
|---|---|
| Volume | 416 |
| ISSN (Print) | 0076-6879 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
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Cite this
Patnaik, S. K., & Stanley, P. (2006). Lectin-Resistant CHO Glycosylation Mutants. In M. Fukuda (Ed.), Glycomics (pp. 159-182). (Methods in Enzymology; Vol. 416). https://doi.org/10.1016/S0076-6879(06)16011-5