Abstract
Chinese hamster ovary (CHO) mutant cells with a wide variety of alterations in the glycosylation of proteins and lipids have been isolated by selection for resistance to the cytotoxicity of plant lectins. These CHO mutants have been used to characterize glycosylation pathways, to identify genes that code for glycosylation activities, to elucidate functional roles of glycans that mediate biological processes, and for glycosylation engineering. In this chapter, we briefly describe the available panel of lectin-resistant CHO mutants and summarize their glycan alterations and the biochemical and genetic bases of mutation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 159-182 |
| Number of pages | 24 |
| Journal | Methods in Enzymology |
| Volume | 416 |
| DOIs | |
| State | Published - 2006 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Biochemistry
Cite this
Lectin-Resistant CHO Glycosylation Mutants. / Patnaik, Santosh Kumar; Stanley, Pamela.
In: Methods in Enzymology, Vol. 416, 2006, p. 159-182.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Lectin-Resistant CHO Glycosylation Mutants
AU - Patnaik, Santosh Kumar
AU - Stanley, Pamela
PY - 2006
Y1 - 2006
N2 - Chinese hamster ovary (CHO) mutant cells with a wide variety of alterations in the glycosylation of proteins and lipids have been isolated by selection for resistance to the cytotoxicity of plant lectins. These CHO mutants have been used to characterize glycosylation pathways, to identify genes that code for glycosylation activities, to elucidate functional roles of glycans that mediate biological processes, and for glycosylation engineering. In this chapter, we briefly describe the available panel of lectin-resistant CHO mutants and summarize their glycan alterations and the biochemical and genetic bases of mutation.
AB - Chinese hamster ovary (CHO) mutant cells with a wide variety of alterations in the glycosylation of proteins and lipids have been isolated by selection for resistance to the cytotoxicity of plant lectins. These CHO mutants have been used to characterize glycosylation pathways, to identify genes that code for glycosylation activities, to elucidate functional roles of glycans that mediate biological processes, and for glycosylation engineering. In this chapter, we briefly describe the available panel of lectin-resistant CHO mutants and summarize their glycan alterations and the biochemical and genetic bases of mutation.
UR - http://www.scopus.com/inward/record.url?scp=33751002037&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33751002037&partnerID=8YFLogxK
U2 - 10.1016/S0076-6879(06)16011-5
DO - 10.1016/S0076-6879(06)16011-5
M3 - Article
C2 - 17113866
AN - SCOPUS:33751002037
VL - 416
SP - 159
EP - 182
JO - ImmunoMethods
JF - ImmunoMethods
SN - 1046-2023
ER -