TY - CHAP
T1 - Lectin-Resistant CHO Glycosylation Mutants
AU - Patnaik, Santosh Kumar
AU - Stanley, Pamela
PY - 2006/11/20
Y1 - 2006/11/20
N2 - Chinese hamster ovary (CHO) mutant cells with a wide variety of alterations in the glycosylation of proteins and lipids have been isolated by selection for resistance to the cytotoxicity of plant lectins. These CHO mutants have been used to characterize glycosylation pathways, to identify genes that code for glycosylation activities, to elucidate functional roles of glycans that mediate biological processes, and for glycosylation engineering. In this chapter, we briefly describe the available panel of lectin-resistant CHO mutants and summarize their glycan alterations and the biochemical and genetic bases of mutation.
AB - Chinese hamster ovary (CHO) mutant cells with a wide variety of alterations in the glycosylation of proteins and lipids have been isolated by selection for resistance to the cytotoxicity of plant lectins. These CHO mutants have been used to characterize glycosylation pathways, to identify genes that code for glycosylation activities, to elucidate functional roles of glycans that mediate biological processes, and for glycosylation engineering. In this chapter, we briefly describe the available panel of lectin-resistant CHO mutants and summarize their glycan alterations and the biochemical and genetic bases of mutation.
UR - http://www.scopus.com/inward/record.url?scp=33751002037&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33751002037&partnerID=8YFLogxK
U2 - 10.1016/S0076-6879(06)16011-5
DO - 10.1016/S0076-6879(06)16011-5
M3 - Chapter
C2 - 17113866
AN - SCOPUS:33751002037
SN - 0121828212
SN - 9780121828219
T3 - Methods in Enzymology
SP - 159
EP - 182
BT - Glycomics
A2 - Fukuda, Minoru
ER -