Lectin-resistant CHO cells

Selection of four new pea lectin-resistant phenotypes

James Ripka, Pamela Stanley

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The cytotoxic plant lectins from P. sativum have been used to select new lectin-resistant mutants from Chinese hamster ovary (CHO) cells. Two novel phenotypes that behave recessively but fall into the same complementation group have been termed Lec 13 and Lec 13A. Both of these mutant types are phenotypically reverted to pea lectin sensitivity following growth in l-fucose. In contrast, two other unique phenotypes behave dominantly in somatic cell hybrids and maintain their pea lectin-resistance in the presence of l-fucose. They have been termed LEC14 and LEC18, respectively. The lectin-resistance and complementation properties of the four new mutant types suggest that they define three different glycosylation genes of the CHO genome.

Original languageEnglish (US)
Pages (from-to)51-62
Number of pages12
JournalSomatic Cell and Molecular Genetics
Volume12
Issue number1
DOIs
StatePublished - Jan 1986

Fingerprint

Fucose
Cricetulus
Lectins
Ovary
Plant Lectins
Phenotype
Hybrid Cells
Glycosylation
Genome
Growth
Genes
pea lectin

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Lectin-resistant CHO cells : Selection of four new pea lectin-resistant phenotypes. / Ripka, James; Stanley, Pamela.

In: Somatic Cell and Molecular Genetics, Vol. 12, No. 1, 01.1986, p. 51-62.

Research output: Contribution to journalArticle

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