Abstract
Chinese hamster ovary (CHO) cells previously selected in a single-step for resistance to one or two different lectins and assigned to individual phenotypic groups on the basis of their unique patterns of lectin resistance, have been examined for their lectin-binding abilites. The lectin-binding parameters of CHO cells were shown to be very complex in a detailes study of the binding of 125I-WGA to wild-type (WT) cells. On the basis of these results, standard assay conditions were established and comparative binding studies between the twenty-two WT and lectin-resistant (LecR) clones were performed. A general correlation of lectin resistance with decreased lectin-binding ability and of lectin sensitivity with increased lectin-binding ability was found, although several exceptions to this trend were observed.
Original language | English (US) |
---|---|
Pages (from-to) | 265-284 |
Number of pages | 20 |
Journal | Advances in Experimental Medicine and Biology |
Volume | 84 |
State | Published - 1977 |
Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Lectin receptors and lectin resistance in chinese hamster ovary cells. / Stanley, Pamela; Carver, J. P.
In: Advances in Experimental Medicine and Biology, Vol. 84, 1977, p. 265-284.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Lectin receptors and lectin resistance in chinese hamster ovary cells.
AU - Stanley, Pamela
AU - Carver, J. P.
PY - 1977
Y1 - 1977
N2 - Chinese hamster ovary (CHO) cells previously selected in a single-step for resistance to one or two different lectins and assigned to individual phenotypic groups on the basis of their unique patterns of lectin resistance, have been examined for their lectin-binding abilites. The lectin-binding parameters of CHO cells were shown to be very complex in a detailes study of the binding of 125I-WGA to wild-type (WT) cells. On the basis of these results, standard assay conditions were established and comparative binding studies between the twenty-two WT and lectin-resistant (LecR) clones were performed. A general correlation of lectin resistance with decreased lectin-binding ability and of lectin sensitivity with increased lectin-binding ability was found, although several exceptions to this trend were observed.
AB - Chinese hamster ovary (CHO) cells previously selected in a single-step for resistance to one or two different lectins and assigned to individual phenotypic groups on the basis of their unique patterns of lectin resistance, have been examined for their lectin-binding abilites. The lectin-binding parameters of CHO cells were shown to be very complex in a detailes study of the binding of 125I-WGA to wild-type (WT) cells. On the basis of these results, standard assay conditions were established and comparative binding studies between the twenty-two WT and lectin-resistant (LecR) clones were performed. A general correlation of lectin resistance with decreased lectin-binding ability and of lectin sensitivity with increased lectin-binding ability was found, although several exceptions to this trend were observed.
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UR - http://www.scopus.com/inward/citedby.url?scp=0017348227&partnerID=8YFLogxK
M3 - Article
C2 - 899950
AN - SCOPUS:0017348227
VL - 84
SP - 265
EP - 284
JO - Advances in Experimental Medicine and Biology
JF - Advances in Experimental Medicine and Biology
SN - 0065-2598
ER -