Lead (Pb) exposure induces dopaminergic neurotoxicity in Caenorhabditis elegans

Involvement of the dopamine transporter

Ayodele Jacob Akinyemi, Mahfuzur R. Miah, Omamuyovwi M. Ijomone, Aristidis Tsatsakis, Félix Alexandre Antunes Soares, Alexey A. Tinkov, Anatoly V. Skalny, Vivek Venkataramani, Michael Aschner

Research output: Contribution to journalArticle

Abstract

Lead (Pb) is an environmental neurotoxicant, and has been implicated in several neurological disorders of dopaminergic dysfunction; however, the molecular mechanism of its toxicity has yet to be fully understood. This study investigated the effect of Pb exposure on dopaminergic neurodegeneration and function, as well as expression level of several dopaminergic signaling genes in wild type (N2) and protein kinase C (pkc) mutant Caenorhabditis elegans. Both N2 and pkc mutant worms were exposed to Pb2+ for 1 h. Thereafter, dopaminergic (DAergic) neurodegeneration, behavior and gene expression levels were assessed. The results revealed that Pb2+ treatment affects dopaminergic cell morphology and structure in worms expressing green fluorescent protein (GFP) under a DAergic cell specific promoter. Also, there was a significant impairment in dopaminergic neuronal function as tested by basal slowing response (BSR) in wild-type, N2 worms, but no effect was observed in pkc mutant worms. Furthermore, Pb2+ exposure increased dat-1 gene expression level when compared with N2 worms, but no alteration was observed in the pkc mutant strains. LC–MS analysis revealed a significant decrease in dopamine content in worms treated with Pb2+ when compared with controls. In summary, our results revealed that Pb2+ exposure induced dopaminergic dysfunction in C. elegans by altering dat-1 gene levels, but pkc mutants showed significant resistance to Pb2+ toxicity. We conclude that PKC activation is directly involved in the neurotoxicity of Pb.

Original languageEnglish (US)
Pages (from-to)833-840
Number of pages8
JournalToxicology Reports
Volume6
DOIs
StatePublished - Jan 1 2019

Fingerprint

Dopamine Plasma Membrane Transport Proteins
Caenorhabditis elegans
Protein Kinase C
Gene expression
Toxicity
Genes
Gene Expression
Green Fluorescent Proteins
Nervous System Diseases
Dopamine
Chemical activation
Lead
Proteins

Keywords

  • dat-1
  • Dopaminergic neuron
  • Neurotoxicant
  • Pb
  • PKC

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Lead (Pb) exposure induces dopaminergic neurotoxicity in Caenorhabditis elegans : Involvement of the dopamine transporter. / Akinyemi, Ayodele Jacob; Miah, Mahfuzur R.; Ijomone, Omamuyovwi M.; Tsatsakis, Aristidis; Soares, Félix Alexandre Antunes; Tinkov, Alexey A.; Skalny, Anatoly V.; Venkataramani, Vivek; Aschner, Michael.

In: Toxicology Reports, Vol. 6, 01.01.2019, p. 833-840.

Research output: Contribution to journalArticle

Akinyemi, AJ, Miah, MR, Ijomone, OM, Tsatsakis, A, Soares, FAA, Tinkov, AA, Skalny, AV, Venkataramani, V & Aschner, M 2019, 'Lead (Pb) exposure induces dopaminergic neurotoxicity in Caenorhabditis elegans: Involvement of the dopamine transporter', Toxicology Reports, vol. 6, pp. 833-840. https://doi.org/10.1016/j.toxrep.2019.08.001
Akinyemi, Ayodele Jacob ; Miah, Mahfuzur R. ; Ijomone, Omamuyovwi M. ; Tsatsakis, Aristidis ; Soares, Félix Alexandre Antunes ; Tinkov, Alexey A. ; Skalny, Anatoly V. ; Venkataramani, Vivek ; Aschner, Michael. / Lead (Pb) exposure induces dopaminergic neurotoxicity in Caenorhabditis elegans : Involvement of the dopamine transporter. In: Toxicology Reports. 2019 ; Vol. 6. pp. 833-840.
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AU - Soares, Félix Alexandre Antunes

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