LC3-dependent intracellular membrane tubules induced by γ-protocadherins A3 and B2: A role for intraluminal interactions

Hugo H. Hanson, Semie Kang, Mónica Fernández-Monreal, Twethida Oung, Murat Yildirim, Rebecca Lee, Kimita Suyama, Rachel Hazan, Greg R. Phillips

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Clustered protocadherins (Pcdhs) are a family of cadherin-like molecules arranged in gene clusters (α, β, and γ). γ-Protocadherins (Pcdh-γs) are involved in cell-cell interactions, but their prominent intracellular distribution in vivo and different knock-out phenotypes suggest that these molecules participate in still unidentified processes. We found using correlative light and electron microscopy that Pcdh-γA3 and -γB2, but not -γC4, -α1, or N-cadherin, generate intracellular juxtanuclear membrane tubules when expressed in cells. These tubules recruit the autophagy marker MAP1A/1B LC3 (LC3) but are not associated with autophagic vesicles. Lipidation of LC3 is required for its coclustering with Pcdh-γtubules, suggesting the involvement of an autophagic-like molecular cascade. Expression of wild-type LC3 with Pcdh-γA3 increased tubule length whereas expression of lipidation-defective LC3 decreased tubule length relative to Pcdh-γA3 expressed alone. The tubules were found to emanate from lysosomes. Deletion of the luminal/extracellular domain of Pcdh-γA3 preserved lysosomal targeting but eliminated tubule formation whereas cytoplasmic deletion eliminated both lysosomal targeting and tubule formation. Deletion of the membrane-proximal three cadherin repeats resulted in tubes that were narrower than those produced by full-length molecules. These results suggest that Pcdh-γA and -γB families can influence the shape of intracellular membranes by mediating intraluminal interactions within organelles.

Original languageEnglish (US)
Pages (from-to)20982-20992
Number of pages11
JournalJournal of Biological Chemistry
Volume285
Issue number27
DOIs
StatePublished - Jul 2 2010

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Intracellular Membranes
Cadherins
Membranes
Molecules
Autophagy
Multigene Family
Lysosomes
Cell Communication
Organelles
Electron microscopy
Optical microscopy
Electron Microscopy
Genes
Phenotype
Light

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Hanson, H. H., Kang, S., Fernández-Monreal, M., Oung, T., Yildirim, M., Lee, R., ... Phillips, G. R. (2010). LC3-dependent intracellular membrane tubules induced by γ-protocadherins A3 and B2: A role for intraluminal interactions. Journal of Biological Chemistry, 285(27), 20982-20992. https://doi.org/10.1074/jbc.M109.092031

LC3-dependent intracellular membrane tubules induced by γ-protocadherins A3 and B2 : A role for intraluminal interactions. / Hanson, Hugo H.; Kang, Semie; Fernández-Monreal, Mónica; Oung, Twethida; Yildirim, Murat; Lee, Rebecca; Suyama, Kimita; Hazan, Rachel; Phillips, Greg R.

In: Journal of Biological Chemistry, Vol. 285, No. 27, 02.07.2010, p. 20982-20992.

Research output: Contribution to journalArticle

Hanson, Hugo H. ; Kang, Semie ; Fernández-Monreal, Mónica ; Oung, Twethida ; Yildirim, Murat ; Lee, Rebecca ; Suyama, Kimita ; Hazan, Rachel ; Phillips, Greg R. / LC3-dependent intracellular membrane tubules induced by γ-protocadherins A3 and B2 : A role for intraluminal interactions. In: Journal of Biological Chemistry. 2010 ; Vol. 285, No. 27. pp. 20982-20992.
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