Laryngeal papilloma cells have high levels of epidermal growth factor receptor and respond to epidermal growth factor by a decrease in epithelial differentiation

Andrea Vambutas, Teresa P. DiLorenzo, Bettie M. Steinberg

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Abstract

Laryngeal papillomas are benign epithelial tumors caused by human papillomaviruses. These tumors are characterized by hyperplasia of the spinous layer and abnormal differentiation. Many tumor cell lines overexpress the epidermal growth factor (EGF) receptor on their surface, and EGF regulates normal cell growth. We have asked about the relationship of the EGF receptor and EGF response in laryngeal papilloma cells. Papilloma cells showed markedly greater immunohistochemical staining for the EGF receptor, compared to uninfected cells. Both cell types showed a 2-3-fold increase in nuclei incorporating bromodeoxyuridine when EGF was present. Removal of EGF from papilloma cells cultured on collagen rafts permitted normal stratification and differentiation, as determined by synthesis of keratin 13. Inclusion of EGF induced abnormal differentiation with minimal expression of keratin 13. Uninfected laryngeal cells cultured on rafts in the presence of EGF synthesize keratin 13 in all suprabasal cells. EGF reduced both human papillomavirus RNA levels in the papilloma cells and expression of a reporter gene linked to the human papillomavirus 11 enhancers and E6 promoter in uninfected cells. These results suggest that the phenotype of papillomas is induced, in part, by EGF binding to the abundant EGF receptors.

Original languageEnglish (US)
Pages (from-to)910-914
Number of pages5
JournalCancer Research
Volume53
Issue number4
StatePublished - Feb 15 1993

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Papilloma
Epidermal Growth Factor Receptor
Epidermal Growth Factor
Keratin-13
Cultured Cells
Human papillomavirus 11
Bromodeoxyuridine
Tumor Cell Line
Reporter Genes
Hyperplasia
Neoplasms
Collagen
RNA
Staining and Labeling
Phenotype
Growth

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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Laryngeal papilloma cells have high levels of epidermal growth factor receptor and respond to epidermal growth factor by a decrease in epithelial differentiation. / Vambutas, Andrea; DiLorenzo, Teresa P.; Steinberg, Bettie M.

In: Cancer Research, Vol. 53, No. 4, 15.02.1993, p. 910-914.

Research output: Contribution to journalArticle

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