Large-scale production of embryonic red blood cells from human embryonic stem cells

Emmanuel N. Olivier, Caihong Qiu, Michelle Velho, Rhoda Elison Hirsch, Eric E. Bouhassira

Research output: Contribution to journalArticle

116 Citations (Scopus)

Abstract

Objective: To develop a method to produce in culture large number of erythroid cells from human embryonic stem cells. Materials and Methods: Human H1 embryonic stem cells were differentiated into hematopoietic cells by coculture with a human fetal liver cell line, and the resulting CD34-positive cells were expanded in vitro in liquid culture using a three-step method. The erythroid cells produced were then analyzed by light microscopy and flow cytometry. Globin expression was characterized by quantitative reverse-transcriptase polymerase chain reaction and by high-performance liquid chromatography. Results: CD34-positive cells produced from human embryonic stem cells could be efficiently differentiated into erythroid cells in liquid culture leading to a more than 5000-fold increase in cell number. The erythroid cells produced are similar to primitive erythroid cells present in the yolk sac of early human embryos and did not enucleate. They are fully hemoglobinized and express a mixture of embryonic and fetal globins but no β-globin. Conclusions: We have developed an experimental protocol to produce large numbers of primitive erythroid cells starting from undifferentiated human embryonic stem cells. As the earliest human erythroid cells, the nucleated primitive erythroblasts, are not very well characterized because experimental material at this stage of development is very difficult to obtain, this system should prove useful to answer a number of experimental questions regarding the biology of these cells. In addition, production of mature red blood cells from human embryonic stem cells is of great potential practical importance because it could eventually become an alternate source of cell for transfusion.

Original languageEnglish (US)
Pages (from-to)1635-1642
Number of pages8
JournalExperimental Hematology
Volume34
Issue number12
DOIs
StatePublished - Dec 2006

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Erythroid Cells
Erythrocytes
Globins
Erythroblasts
Yolk Sac
Coculture Techniques
Human Embryonic Stem Cells
Reverse Transcriptase Polymerase Chain Reaction
Cell Biology
Microscopy
Flow Cytometry
Embryonic Structures
Cell Count
High Pressure Liquid Chromatography
Light
Cell Line
Liver

ASJC Scopus subject areas

  • Cancer Research
  • Cell Biology
  • Genetics
  • Hematology
  • Oncology
  • Transplantation

Cite this

Large-scale production of embryonic red blood cells from human embryonic stem cells. / Olivier, Emmanuel N.; Qiu, Caihong; Velho, Michelle; Hirsch, Rhoda Elison; Bouhassira, Eric E.

In: Experimental Hematology, Vol. 34, No. 12, 12.2006, p. 1635-1642.

Research output: Contribution to journalArticle

Olivier, Emmanuel N. ; Qiu, Caihong ; Velho, Michelle ; Hirsch, Rhoda Elison ; Bouhassira, Eric E. / Large-scale production of embryonic red blood cells from human embryonic stem cells. In: Experimental Hematology. 2006 ; Vol. 34, No. 12. pp. 1635-1642.
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