Langerin negative dendritic cells promote potent CD8+ T-cell priming by skin delivery of live adenovirus vaccine microneedle arrays

Veronique Bachy, Catherine Hervouet, Pablo D. Becker, Laurent Chorro, Leo M. Carlin, Shanthi Herath, Timos Papagatsias, Jean Baptiste Barbaroux, Sea Jin Oh, Adel Benlahrech, Takis Athanasopoulos, George Dickson, Steven Patterson, Sung Yun Kwon, Frederic Geissmann, Linda S. Klavinskis

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Stabilization of virus protein structure and nucleic acid integrity is challenging yet essential to preserve the transcriptional competence of live recombinant viral vaccine vectors in the absence of a cold chain. When coupled with needle-free skin delivery, such a platform would address an unmet need in global vaccine coverage against HIV and other global pathogens. Herein, we show that a simple dissolvable microneedle array (MA) delivery system preserves the immunogenicity of vaccines encoded by live recombinant human adenovirus type 5 (rAdHu5). Specifically, dried rAdHu5MAimmunizationinduced CD8+ T-cell expansion and multifunctional cytokine responses equipotent with conventional injectable routes of immunization. Intravital imagingdemonstratedMAcargodistributed both in the epidermis and dermis, with acquisition by CD11c+ dendritic cells (DCs) in the dermis. The MA immunizing properties were attributable to CD11c+ MHCIIhi CD8αneg epithelial cell adhesion molecule (EpCAMneg) CD11b+ langerin (Lang; CD207)neg DCs, but neither Langerhans cells nor Lang+ DCswere required for CD8+ T-cell priming. This study demonstrates an important technical advance for viral vaccine vectors progressing to the clinic and provides insights into the mechanism of CD8+ T-cell priming by live rAdHu5 MAs.

Original languageEnglish (US)
Pages (from-to)3041-3046
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number8
DOIs
StatePublished - Feb 19 2013
Externally publishedYes

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Adenovirus Vaccines
Viral Vaccines
Dendritic Cells
Dermis
T-Lymphocytes
Skin
Human Adenoviruses
Viral Structures
Refrigeration
Synthetic Vaccines
Langerhans Cells
Epidermis
Mental Competency
Nucleic Acids
Needles
Immunization
Vaccines
HIV
Cytokines
Injections

ASJC Scopus subject areas

  • General

Cite this

Langerin negative dendritic cells promote potent CD8+ T-cell priming by skin delivery of live adenovirus vaccine microneedle arrays. / Bachy, Veronique; Hervouet, Catherine; Becker, Pablo D.; Chorro, Laurent; Carlin, Leo M.; Herath, Shanthi; Papagatsias, Timos; Barbaroux, Jean Baptiste; Oh, Sea Jin; Benlahrech, Adel; Athanasopoulos, Takis; Dickson, George; Patterson, Steven; Kwon, Sung Yun; Geissmann, Frederic; Klavinskis, Linda S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, No. 8, 19.02.2013, p. 3041-3046.

Research output: Contribution to journalArticle

Bachy, V, Hervouet, C, Becker, PD, Chorro, L, Carlin, LM, Herath, S, Papagatsias, T, Barbaroux, JB, Oh, SJ, Benlahrech, A, Athanasopoulos, T, Dickson, G, Patterson, S, Kwon, SY, Geissmann, F & Klavinskis, LS 2013, 'Langerin negative dendritic cells promote potent CD8+ T-cell priming by skin delivery of live adenovirus vaccine microneedle arrays', Proceedings of the National Academy of Sciences of the United States of America, vol. 110, no. 8, pp. 3041-3046. https://doi.org/10.1073/pnas.1214449110
Bachy, Veronique ; Hervouet, Catherine ; Becker, Pablo D. ; Chorro, Laurent ; Carlin, Leo M. ; Herath, Shanthi ; Papagatsias, Timos ; Barbaroux, Jean Baptiste ; Oh, Sea Jin ; Benlahrech, Adel ; Athanasopoulos, Takis ; Dickson, George ; Patterson, Steven ; Kwon, Sung Yun ; Geissmann, Frederic ; Klavinskis, Linda S. / Langerin negative dendritic cells promote potent CD8+ T-cell priming by skin delivery of live adenovirus vaccine microneedle arrays. In: Proceedings of the National Academy of Sciences of the United States of America. 2013 ; Vol. 110, No. 8. pp. 3041-3046.
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