Laminar and temporal heterogeneity of NMDA/metabotropic glutamate receptor binding in posterior cingulate cortex

Thomas G. Hedberg, Ellen F. Sperber, Jana Veĺǐsková, Solomon L. Moshe

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Both N-methyl-D-aspartate (NMDA) and quisqualate/AMPA-insensitive metabotropic glutamate (mGlu) receptors mediate plasticity induction in neocortex, but their interlaminar distribution in cortical microcircuits is largely unknown. We used (+)3H-MK801 and 3H-glutamate binding plus saturating concentrations of NMDA, AMPA, and quisqualate to autoradiographically map NMDA and mGlu receptor sites by lamina in posterior cingulate cortex in adult male rats. Specific binding at NMDA receptor sites in laminae II/III and VI was significantly reduced in comparison to other laminae. Brains prepared from rats killed during dark phase of a 12h/12h light/dark cycle showed a mean 129% increase in overall (+)3H-MK801 binding versus light phase brains but retained reduced binding densities in laminae II/III and VI. In contrast to NMDA findings, specific binding at mGlu sites was consistently elevated during light phase in both laminae II/III and VI. Specific 3H-glutamate binding in dark-phase brains showed an overall 147% increase versus light phase binding but did not retain significant interlaminar heterogeneity. Interpreted in accordance with our physiologically derived models of hippocampo-cortical microcircuitry, these results suggest that spatial and temporal variations in glutamate receptor distribution may play an important role in intracingulate neural processing of afferent input from hippocampus.

Original languageEnglish (US)
Pages (from-to)1881-1887
Number of pages7
JournalJournal of Neurophysiology
Volume84
Issue number4
StatePublished - 2000

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Metabotropic Glutamate Receptors
Gyrus Cinguli
Substantia Gelatinosa
N-Methylaspartate
Quisqualic Acid
Glutamic Acid
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Light
Brain
Neocortex
Photoperiod
Glutamate Receptors
N-Methyl-D-Aspartate Receptors
Hippocampus

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

Cite this

Laminar and temporal heterogeneity of NMDA/metabotropic glutamate receptor binding in posterior cingulate cortex. / Hedberg, Thomas G.; Sperber, Ellen F.; Veĺǐsková, Jana; Moshe, Solomon L.

In: Journal of Neurophysiology, Vol. 84, No. 4, 2000, p. 1881-1887.

Research output: Contribution to journalArticle

Hedberg, Thomas G. ; Sperber, Ellen F. ; Veĺǐsková, Jana ; Moshe, Solomon L. / Laminar and temporal heterogeneity of NMDA/metabotropic glutamate receptor binding in posterior cingulate cortex. In: Journal of Neurophysiology. 2000 ; Vol. 84, No. 4. pp. 1881-1887.
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