Lack of responsiveness of C3H/HeJ macrophages to lipopolysaccharide: The cellular basis of LPS-stimulated metabolism

J. L. Ryan, L. M. Glode, D. L. Rosenstreich

Research output: Contribution to journalArticle

46 Scopus citations


The rate of glucose utilization has been used as a measure of LPS-induced activation of cultures of C3H/HeN and C3H/HeJ spleen cells, peritoneal cells, and purified peritoneal adherent cells. Peritoneal cells utilized 40 to 60 times more glucose than did spleen cells and purified adherent monolayers were more active than mixed peritoneal cells, suggesting that only macrophage metabolism was being measured. The cell preparations for C3H/HeJ mice were not activated by Escherichia coli K235 LPS prepared by extensive phenol extraction, whereas C3H/HeN cells were activated by that LPS. Cells from both strains were activated by a commercially obtained E. coli 0111:B4 LPS and butanol-extracted K235 LPS. The addition of 10% C3H/HeN spleen cells to C3H/HeJ peritoneal cells resulted in a marked enhancement of glucose utilization. These findings suggest that LPS-induced enhancement of macrophage metabolism occurs both by direct action of LPS on macrophages as well as indirectly through activated lymphocytes.

Original languageEnglish (US)
Pages (from-to)932-935
Number of pages4
JournalJournal of Immunology
Issue number3
Publication statusPublished - Jan 1 1979
Externally publishedYes


ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this