Lack of correlation of functional scintigraphy with 99mtechnetium-methoxyisobutylisonitrile with histological necrosis following induction chemotherapy or measures of P-glycoprotein expression in high-grade osteosarcoma

Richard Gorlick, Rebecca Sowers, Mariza Daras, Elizabeth Calleja, Leonard H. Wexler, Paul A. Meyers, Alfred C. Liao, Samuel D. Yeh, Steven M. Larson, Cristina Antonescu, Andrew G. Huvos, David Panicek, John H. Healey

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21 Scopus citations

Abstract

In osteosarcoma, some studies have suggested P-glycoprotein expression is a prognostic factor. The clearance of 99mtechnetium hexakis-2-methoxyisobutylisonitrile (99mTc-MIBI) has been used in some tumor systems as an in vivo measure of P-glycoprotein-mediated efflux. In this study we explored the correlation between 99mTc-MIBI clearance and histological necrosis following induction chemotherapy and P-glycoprotein expression in osteosarcoma. The primary tumors of 20 patients with high-grade osteosarcoma were imaged at diagnosis with 99mTc-MIBI, and the uptake ratios and biological half-lives were calculated. P-Glycoprotein expression in the tumor tissue was determined immunohistochemically and by measuring mRNA expression of the multidrug resistance-1 gene. The histological necrosis following induction chemotherapy was assessed by the Huvos grading system. The biological half-life of 99mTc-MIBI ranged from 1.4 to 52.5 h. Seven of the 20 tumor samples had a favorable extent of necrosis following induction chemotherapy. The 99mTc-MIBI half-life and uptake ratio showed no correlation with histological necrosis following induction chemotherapy. The 99mTc-MIBI half-life and uptake ratio did not correlate with either measure of P-glycoprotein expression. The results of this pilot study indicate that 99mTc-MIBI imaging is not an effective predictor of histological necrosis following induction chemotherapy in high-grade osteosarcoma. 99mTc-MIBI imaging did not correlate with measures of P-glycoprotein expression in the tumor tissue.

Original languageEnglish (US)
Pages (from-to)3065-3070
Number of pages6
JournalClinical Cancer Research
Volume7
Issue number10
StatePublished - Jan 1 2001

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Gorlick, R., Sowers, R., Daras, M., Calleja, E., Wexler, L. H., Meyers, P. A., Liao, A. C., Yeh, S. D., Larson, S. M., Antonescu, C., Huvos, A. G., Panicek, D., & Healey, J. H. (2001). Lack of correlation of functional scintigraphy with 99mtechnetium-methoxyisobutylisonitrile with histological necrosis following induction chemotherapy or measures of P-glycoprotein expression in high-grade osteosarcoma. Clinical Cancer Research, 7(10), 3065-3070.