Labeling tumor cells with fluorescent nanocrystal-aptamer bioconjugates

Ted C. Chu, Felice Shieh, Laura A. Lavery, Matthew Levy, Rebecca Richards-Kortum, Brian A. Korgel, Andrew D. Ellington

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

Aptamers that bind to prostate specific membrane antigen (PSMA) were conjugated to luminescent CdSe and CdTe nanocrystals for cell-labeling studies. The aptamer-nanocrystal conjugates showed specific targeting of both fixed and live cells that overexpressed PSMA. More importantly, aptamers were able to label cells dispersed in a collagen gel matrix simulating tissue. The specific binding abilities and synthetic accessibility of aptamers combined with the photostability and small size of semiconductor nanocrystals offers a powerful and general tool for cellular imaging.

Original languageEnglish (US)
Pages (from-to)1859-1866
Number of pages8
JournalBiosensors and Bioelectronics
Volume21
Issue number10
DOIs
StatePublished - Apr 15 2006
Externally publishedYes

Fingerprint

Nanoparticles
Nanocrystals
Labeling
Tumors
Cells
Antigens
Membranes
Quantum Dots
Neoplasms
Collagen
Labels
Gels
Tissue
Semiconductor materials
Imaging techniques
human glutamate carboxypeptidase II

Keywords

  • Aptamer
  • Cell imaging
  • Nanocrystal

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Electrochemistry

Cite this

Chu, T. C., Shieh, F., Lavery, L. A., Levy, M., Richards-Kortum, R., Korgel, B. A., & Ellington, A. D. (2006). Labeling tumor cells with fluorescent nanocrystal-aptamer bioconjugates. Biosensors and Bioelectronics, 21(10), 1859-1866. https://doi.org/10.1016/j.bios.2005.12.015

Labeling tumor cells with fluorescent nanocrystal-aptamer bioconjugates. / Chu, Ted C.; Shieh, Felice; Lavery, Laura A.; Levy, Matthew; Richards-Kortum, Rebecca; Korgel, Brian A.; Ellington, Andrew D.

In: Biosensors and Bioelectronics, Vol. 21, No. 10, 15.04.2006, p. 1859-1866.

Research output: Contribution to journalArticle

Chu, TC, Shieh, F, Lavery, LA, Levy, M, Richards-Kortum, R, Korgel, BA & Ellington, AD 2006, 'Labeling tumor cells with fluorescent nanocrystal-aptamer bioconjugates', Biosensors and Bioelectronics, vol. 21, no. 10, pp. 1859-1866. https://doi.org/10.1016/j.bios.2005.12.015
Chu TC, Shieh F, Lavery LA, Levy M, Richards-Kortum R, Korgel BA et al. Labeling tumor cells with fluorescent nanocrystal-aptamer bioconjugates. Biosensors and Bioelectronics. 2006 Apr 15;21(10):1859-1866. https://doi.org/10.1016/j.bios.2005.12.015
Chu, Ted C. ; Shieh, Felice ; Lavery, Laura A. ; Levy, Matthew ; Richards-Kortum, Rebecca ; Korgel, Brian A. ; Ellington, Andrew D. / Labeling tumor cells with fluorescent nanocrystal-aptamer bioconjugates. In: Biosensors and Bioelectronics. 2006 ; Vol. 21, No. 10. pp. 1859-1866.
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