Labeling tumor cells with fluorescent nanocrystal-aptamer bioconjugates

Ted C. Chu, Felice Shieh, Laura A. Lavery, Matthew Levy, Rebecca Richards-Kortum, Brian A. Korgel, Andrew D. Ellington

Research output: Contribution to journalArticle

114 Scopus citations

Abstract

Aptamers that bind to prostate specific membrane antigen (PSMA) were conjugated to luminescent CdSe and CdTe nanocrystals for cell-labeling studies. The aptamer-nanocrystal conjugates showed specific targeting of both fixed and live cells that overexpressed PSMA. More importantly, aptamers were able to label cells dispersed in a collagen gel matrix simulating tissue. The specific binding abilities and synthetic accessibility of aptamers combined with the photostability and small size of semiconductor nanocrystals offers a powerful and general tool for cellular imaging.

Original languageEnglish (US)
Pages (from-to)1859-1866
Number of pages8
JournalBiosensors and Bioelectronics
Volume21
Issue number10
DOIs
StatePublished - Apr 15 2006

Keywords

  • Aptamer
  • Cell imaging
  • Nanocrystal

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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    Chu, T. C., Shieh, F., Lavery, L. A., Levy, M., Richards-Kortum, R., Korgel, B. A., & Ellington, A. D. (2006). Labeling tumor cells with fluorescent nanocrystal-aptamer bioconjugates. Biosensors and Bioelectronics, 21(10), 1859-1866. https://doi.org/10.1016/j.bios.2005.12.015