TY - CHAP
T1 - Labeling stem cells with superparamagnetic iron oxide nanoparticles
T2 - Analysis of the labeling efficacy by microscopy and magnetic resonance imaging
AU - Jasmin,
AU - Torres, Ana Luiza Mac Hado
AU - Jelicks, Linda
AU - De Carvalho, Antonio Carlos Campos
AU - Spray, David C.
AU - Mendez-Otero, Rosalia
PY - 2012
Y1 - 2012
N2 - Stem cell therapy has emerged as a potential therapeutic option for cell death-related heart diseases. Application of non-invasive cell tracking approaches is necessary to determine tissue distribution and lifetime of stem cells following their injection and will likely provide knowledge about poorly understood stem cells mechanisms of tissue repair. Magnetic resonance imaging (MRI) is a potentially excellent tool for high-resolution visualization of the fate of cells after transplantation and for evaluation of therapeutic strategies. The application of MRI for in vivo cell tracking requires contrast agents to achieve efficient cell labeling without causing any toxic cellular effects or eliciting any other side effects. For these reasons clinically approved contrast agents (e.g., ferumoxides) and incorporation facilitators (e.g., protamine) are currently the preferred materials for cell labeling and tracking. Here we describe how to use superparamagnetic iron oxide nanoparticles to label cells and to monitor cell fate in several disease models.
AB - Stem cell therapy has emerged as a potential therapeutic option for cell death-related heart diseases. Application of non-invasive cell tracking approaches is necessary to determine tissue distribution and lifetime of stem cells following their injection and will likely provide knowledge about poorly understood stem cells mechanisms of tissue repair. Magnetic resonance imaging (MRI) is a potentially excellent tool for high-resolution visualization of the fate of cells after transplantation and for evaluation of therapeutic strategies. The application of MRI for in vivo cell tracking requires contrast agents to achieve efficient cell labeling without causing any toxic cellular effects or eliciting any other side effects. For these reasons clinically approved contrast agents (e.g., ferumoxides) and incorporation facilitators (e.g., protamine) are currently the preferred materials for cell labeling and tracking. Here we describe how to use superparamagnetic iron oxide nanoparticles to label cells and to monitor cell fate in several disease models.
KW - Cell labeling
KW - Ferumoxides
KW - Magnetic resonance imaging
KW - Stem cells
KW - Superparamagnetic iron oxide nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=84864862402&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84864862402&partnerID=8YFLogxK
U2 - 10.1007/978-1-61779-953-2_18
DO - 10.1007/978-1-61779-953-2_18
M3 - Chapter
C2 - 22791437
AN - SCOPUS:84864862402
SN - 9781617799525
T3 - Methods in Molecular Biology
SP - 239
EP - 252
BT - Nanoparticles in Biology and Medicine
A2 - Soloviev, Mikhail
ER -