Labeling stem cells with superparamagnetic iron oxide nanoparticles: Analysis of the labeling efficacy by microscopy and magnetic resonance imaging

Jasmin, Ana Luiza MacHado Torres, Linda Jelicks, Antonio Carlos Campos De Carvalho, David C. Spray, Rosalia Mendez-Otero

Research output: Chapter in Book/Report/Conference proceedingChapter

29 Citations (Scopus)

Abstract

Stem cell therapy has emerged as a potential therapeutic option for cell death-related heart diseases. Application of non-invasive cell tracking approaches is necessary to determine tissue distribution and lifetime of stem cells following their injection and will likely provide knowledge about poorly understood stem cells mechanisms of tissue repair. Magnetic resonance imaging (MRI) is a potentially excellent tool for high-resolution visualization of the fate of cells after transplantation and for evaluation of therapeutic strategies. The application of MRI for in vivo cell tracking requires contrast agents to achieve efficient cell labeling without causing any toxic cellular effects or eliciting any other side effects. For these reasons clinically approved contrast agents (e.g., ferumoxides) and incorporation facilitators (e.g., protamine) are currently the preferred materials for cell labeling and tracking. Here we describe how to use superparamagnetic iron oxide nanoparticles to label cells and to monitor cell fate in several disease models.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages239-252
Number of pages14
Volume906
DOIs
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
Volume906
ISSN (Print)10643745

Fingerprint

Cell Tracking
Nanoparticles
Microscopy
Stem Cells
Magnetic Resonance Imaging
Contrast Media
Protamines
Poisons
Cell Transplantation
Tissue Distribution
Cell- and Tissue-Based Therapy
Heart Diseases
Cell Death
Injections
Therapeutics
ferric oxide

Keywords

  • Cell labeling
  • Ferumoxides
  • Magnetic resonance imaging
  • Stem cells
  • Superparamagnetic iron oxide nanoparticles

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Jasmin, Torres, A. L. M., Jelicks, L., De Carvalho, A. C. C., Spray, D. C., & Mendez-Otero, R. (2012). Labeling stem cells with superparamagnetic iron oxide nanoparticles: Analysis of the labeling efficacy by microscopy and magnetic resonance imaging. In Methods in Molecular Biology (Vol. 906, pp. 239-252). (Methods in Molecular Biology; Vol. 906). https://doi.org/10.1007/978-1-61779-953-2_18

Labeling stem cells with superparamagnetic iron oxide nanoparticles : Analysis of the labeling efficacy by microscopy and magnetic resonance imaging. / Jasmin; Torres, Ana Luiza MacHado; Jelicks, Linda; De Carvalho, Antonio Carlos Campos; Spray, David C.; Mendez-Otero, Rosalia.

Methods in Molecular Biology. Vol. 906 2012. p. 239-252 (Methods in Molecular Biology; Vol. 906).

Research output: Chapter in Book/Report/Conference proceedingChapter

Jasmin, Torres, ALM, Jelicks, L, De Carvalho, ACC, Spray, DC & Mendez-Otero, R 2012, Labeling stem cells with superparamagnetic iron oxide nanoparticles: Analysis of the labeling efficacy by microscopy and magnetic resonance imaging. in Methods in Molecular Biology. vol. 906, Methods in Molecular Biology, vol. 906, pp. 239-252. https://doi.org/10.1007/978-1-61779-953-2_18
Jasmin, Torres ALM, Jelicks L, De Carvalho ACC, Spray DC, Mendez-Otero R. Labeling stem cells with superparamagnetic iron oxide nanoparticles: Analysis of the labeling efficacy by microscopy and magnetic resonance imaging. In Methods in Molecular Biology. Vol. 906. 2012. p. 239-252. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-953-2_18
Jasmin ; Torres, Ana Luiza MacHado ; Jelicks, Linda ; De Carvalho, Antonio Carlos Campos ; Spray, David C. ; Mendez-Otero, Rosalia. / Labeling stem cells with superparamagnetic iron oxide nanoparticles : Analysis of the labeling efficacy by microscopy and magnetic resonance imaging. Methods in Molecular Biology. Vol. 906 2012. pp. 239-252 (Methods in Molecular Biology).
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