Labeling stem cells with superparamagnetic iron oxide nanoparticles: Analysis of the labeling efficacy by microscopy and magnetic resonance imaging

Jasmin, Ana Luiza Mac Hado Torres, Linda Jelicks, Antonio Carlos Campos De Carvalho, David C. Spray, Rosalia Mendez-Otero

Research output: Chapter in Book/Report/Conference proceedingChapter

34 Scopus citations

Abstract

Stem cell therapy has emerged as a potential therapeutic option for cell death-related heart diseases. Application of non-invasive cell tracking approaches is necessary to determine tissue distribution and lifetime of stem cells following their injection and will likely provide knowledge about poorly understood stem cells mechanisms of tissue repair. Magnetic resonance imaging (MRI) is a potentially excellent tool for high-resolution visualization of the fate of cells after transplantation and for evaluation of therapeutic strategies. The application of MRI for in vivo cell tracking requires contrast agents to achieve efficient cell labeling without causing any toxic cellular effects or eliciting any other side effects. For these reasons clinically approved contrast agents (e.g., ferumoxides) and incorporation facilitators (e.g., protamine) are currently the preferred materials for cell labeling and tracking. Here we describe how to use superparamagnetic iron oxide nanoparticles to label cells and to monitor cell fate in several disease models.

Original languageEnglish (US)
Title of host publicationNanoparticles in Biology and Medicine
Subtitle of host publicationMethods and Protocols
EditorsMikhail Soloviev
Pages239-252
Number of pages14
DOIs
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
Volume906
ISSN (Print)1064-3745

Keywords

  • Cell labeling
  • Ferumoxides
  • Magnetic resonance imaging
  • Stem cells
  • Superparamagnetic iron oxide nanoparticles

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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