Labeling of hemoglobin with pyridoxal phosphate

R. Benesch, R. E. Benesch, S. Kwong, A. S. Acharya, J. M. Manning

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The reaction of pyridoxal 5'-phosphate (PLP) with deoxyhemoglobin is confined to 2 residues in the β chains i.e. the α-amino group of valine 1 and the ε-amino group of lysine 82, both of which are located in the polyphosphate binding site. The major product is a hemoglobin in which only the two NH2-terminal amino groups are substituted (symmetric diPLPHb). It is formed by subunit rearrangement of monoPLPHb which is the initial product of the pyridoxylation under anaerobic conditions. TetraPLPHb, with substitutions at lysine 82 and valine 1 of both β chains is found as a minor component. It results from subunit exchange of asymmetric diPLPHb consisting of one unmodified αβ dimer and one which is pyridoxylated at both sites. Anaerobic electrophoresis and oxygenation curves show that this reaction is readily reversed by mixing the tetrasubstituted derivative with unmodified hemoglobin.

Original languageEnglish (US)
Pages (from-to)1320-1324
Number of pages5
JournalJournal of Biological Chemistry
Volume257
Issue number3
StatePublished - 1982
Externally publishedYes

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Pyridoxal Phosphate
Valine
Labeling
Lysine
Hemoglobins
Polyphosphates
Oxygenation
Electrophoresis
Dimers
Substitution reactions
Binding Sites
Derivatives
deoxyhemoglobin

ASJC Scopus subject areas

  • Biochemistry

Cite this

Benesch, R., Benesch, R. E., Kwong, S., Acharya, A. S., & Manning, J. M. (1982). Labeling of hemoglobin with pyridoxal phosphate. Journal of Biological Chemistry, 257(3), 1320-1324.

Labeling of hemoglobin with pyridoxal phosphate. / Benesch, R.; Benesch, R. E.; Kwong, S.; Acharya, A. S.; Manning, J. M.

In: Journal of Biological Chemistry, Vol. 257, No. 3, 1982, p. 1320-1324.

Research output: Contribution to journalArticle

Benesch, R, Benesch, RE, Kwong, S, Acharya, AS & Manning, JM 1982, 'Labeling of hemoglobin with pyridoxal phosphate', Journal of Biological Chemistry, vol. 257, no. 3, pp. 1320-1324.
Benesch R, Benesch RE, Kwong S, Acharya AS, Manning JM. Labeling of hemoglobin with pyridoxal phosphate. Journal of Biological Chemistry. 1982;257(3):1320-1324.
Benesch, R. ; Benesch, R. E. ; Kwong, S. ; Acharya, A. S. ; Manning, J. M. / Labeling of hemoglobin with pyridoxal phosphate. In: Journal of Biological Chemistry. 1982 ; Vol. 257, No. 3. pp. 1320-1324.
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