Label-free proteomic analysis of red blood cell membrane fractions from abdominal aortic aneurysm patients

Roxana Martinez-Pinna, Elena Burillo, Julio Madrigal-Matute, Juan Antonio Lopez, Emilio Camafeita, Monica Maria Torres-Fonseca, Patricia Llamas-Granda, Jesus Egido, Jean Baptiste Michel, Luis Miguel Blanco-Colio, Jose Luis Martin-Ventura

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Purpose: To test whether red blood cell (RBC) membrane composition is modified in abdominal aortic aneurysms (AAA) patients. Experimental design: RBC membrane extracts from AAA patients (aortic diameter >3 cm, n = 7) and control subjects (n = 4) were analyzed by a label-free quantitative MS-based strategy, using spectral count data. Additional validation was performed by western-blot. Results: Data analysis based on spectral count from MS/MS-based experiments provided us a signature of 39 proteins differentially expressed in RBC membranes between AAA and controls (changes equal/over 1.515-fold; p-values equal/lower 0.05). MS data revealed altered levels of structural membrane proteins (e.g. spectrins and ankyrin), components of the degradation machinery (proteasome subunits), and oxidative stress related proteins (e.g. catalase and peroxiredoxin-2) among others. Decreased catalase and peroxiredoxin-2 expression in RBC membrane of AAA patients compared to controls were further validated by Western blot, confirming the proteomic results. Conclusions and clinical relevance: RBCs membrane protein composition is altered in AAA patients, which could be involved in the pathological role of RBCs in aortic tissue and become potential targets to prevent AAA progression.

Original languageEnglish (US)
Pages (from-to)626-630
Number of pages5
JournalProteomics - Clinical Applications
Volume8
Issue number7-8
DOIs
StatePublished - Aug 2014
Externally publishedYes

Fingerprint

Abdominal Aortic Aneurysm
Cell membranes
Proteomics
Labels
Blood
Erythrocytes
Cell Membrane
Peroxiredoxins
Catalase
Membrane Proteins
Ankyrins
Spectrin
Oxidative stress
Proteasome Endopeptidase Complex
Chemical analysis
Far-Western Blotting
Design of experiments
Machinery
Proteins
Tissue

Keywords

  • Abdominal aortic aneurysm
  • Red blood cells

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Martinez-Pinna, R., Burillo, E., Madrigal-Matute, J., Lopez, J. A., Camafeita, E., Torres-Fonseca, M. M., ... Martin-Ventura, J. L. (2014). Label-free proteomic analysis of red blood cell membrane fractions from abdominal aortic aneurysm patients. Proteomics - Clinical Applications, 8(7-8), 626-630. https://doi.org/10.1002/prca.201400035

Label-free proteomic analysis of red blood cell membrane fractions from abdominal aortic aneurysm patients. / Martinez-Pinna, Roxana; Burillo, Elena; Madrigal-Matute, Julio; Lopez, Juan Antonio; Camafeita, Emilio; Torres-Fonseca, Monica Maria; Llamas-Granda, Patricia; Egido, Jesus; Michel, Jean Baptiste; Blanco-Colio, Luis Miguel; Martin-Ventura, Jose Luis.

In: Proteomics - Clinical Applications, Vol. 8, No. 7-8, 08.2014, p. 626-630.

Research output: Contribution to journalArticle

Martinez-Pinna, R, Burillo, E, Madrigal-Matute, J, Lopez, JA, Camafeita, E, Torres-Fonseca, MM, Llamas-Granda, P, Egido, J, Michel, JB, Blanco-Colio, LM & Martin-Ventura, JL 2014, 'Label-free proteomic analysis of red blood cell membrane fractions from abdominal aortic aneurysm patients', Proteomics - Clinical Applications, vol. 8, no. 7-8, pp. 626-630. https://doi.org/10.1002/prca.201400035
Martinez-Pinna, Roxana ; Burillo, Elena ; Madrigal-Matute, Julio ; Lopez, Juan Antonio ; Camafeita, Emilio ; Torres-Fonseca, Monica Maria ; Llamas-Granda, Patricia ; Egido, Jesus ; Michel, Jean Baptiste ; Blanco-Colio, Luis Miguel ; Martin-Ventura, Jose Luis. / Label-free proteomic analysis of red blood cell membrane fractions from abdominal aortic aneurysm patients. In: Proteomics - Clinical Applications. 2014 ; Vol. 8, No. 7-8. pp. 626-630.
@article{1fc7589a64b845d1b5ae3ac9fbf37ae1,
title = "Label-free proteomic analysis of red blood cell membrane fractions from abdominal aortic aneurysm patients",
abstract = "Purpose: To test whether red blood cell (RBC) membrane composition is modified in abdominal aortic aneurysms (AAA) patients. Experimental design: RBC membrane extracts from AAA patients (aortic diameter >3 cm, n = 7) and control subjects (n = 4) were analyzed by a label-free quantitative MS-based strategy, using spectral count data. Additional validation was performed by western-blot. Results: Data analysis based on spectral count from MS/MS-based experiments provided us a signature of 39 proteins differentially expressed in RBC membranes between AAA and controls (changes equal/over 1.515-fold; p-values equal/lower 0.05). MS data revealed altered levels of structural membrane proteins (e.g. spectrins and ankyrin), components of the degradation machinery (proteasome subunits), and oxidative stress related proteins (e.g. catalase and peroxiredoxin-2) among others. Decreased catalase and peroxiredoxin-2 expression in RBC membrane of AAA patients compared to controls were further validated by Western blot, confirming the proteomic results. Conclusions and clinical relevance: RBCs membrane protein composition is altered in AAA patients, which could be involved in the pathological role of RBCs in aortic tissue and become potential targets to prevent AAA progression.",
keywords = "Abdominal aortic aneurysm, Red blood cells",
author = "Roxana Martinez-Pinna and Elena Burillo and Julio Madrigal-Matute and Lopez, {Juan Antonio} and Emilio Camafeita and Torres-Fonseca, {Monica Maria} and Patricia Llamas-Granda and Jesus Egido and Michel, {Jean Baptiste} and Blanco-Colio, {Luis Miguel} and Martin-Ventura, {Jose Luis}",
year = "2014",
month = "8",
doi = "10.1002/prca.201400035",
language = "English (US)",
volume = "8",
pages = "626--630",
journal = "Proteomics - Clinical Applications",
issn = "1862-8346",
publisher = "Wiley-VCH Verlag",
number = "7-8",

}

TY - JOUR

T1 - Label-free proteomic analysis of red blood cell membrane fractions from abdominal aortic aneurysm patients

AU - Martinez-Pinna, Roxana

AU - Burillo, Elena

AU - Madrigal-Matute, Julio

AU - Lopez, Juan Antonio

AU - Camafeita, Emilio

AU - Torres-Fonseca, Monica Maria

AU - Llamas-Granda, Patricia

AU - Egido, Jesus

AU - Michel, Jean Baptiste

AU - Blanco-Colio, Luis Miguel

AU - Martin-Ventura, Jose Luis

PY - 2014/8

Y1 - 2014/8

N2 - Purpose: To test whether red blood cell (RBC) membrane composition is modified in abdominal aortic aneurysms (AAA) patients. Experimental design: RBC membrane extracts from AAA patients (aortic diameter >3 cm, n = 7) and control subjects (n = 4) were analyzed by a label-free quantitative MS-based strategy, using spectral count data. Additional validation was performed by western-blot. Results: Data analysis based on spectral count from MS/MS-based experiments provided us a signature of 39 proteins differentially expressed in RBC membranes between AAA and controls (changes equal/over 1.515-fold; p-values equal/lower 0.05). MS data revealed altered levels of structural membrane proteins (e.g. spectrins and ankyrin), components of the degradation machinery (proteasome subunits), and oxidative stress related proteins (e.g. catalase and peroxiredoxin-2) among others. Decreased catalase and peroxiredoxin-2 expression in RBC membrane of AAA patients compared to controls were further validated by Western blot, confirming the proteomic results. Conclusions and clinical relevance: RBCs membrane protein composition is altered in AAA patients, which could be involved in the pathological role of RBCs in aortic tissue and become potential targets to prevent AAA progression.

AB - Purpose: To test whether red blood cell (RBC) membrane composition is modified in abdominal aortic aneurysms (AAA) patients. Experimental design: RBC membrane extracts from AAA patients (aortic diameter >3 cm, n = 7) and control subjects (n = 4) were analyzed by a label-free quantitative MS-based strategy, using spectral count data. Additional validation was performed by western-blot. Results: Data analysis based on spectral count from MS/MS-based experiments provided us a signature of 39 proteins differentially expressed in RBC membranes between AAA and controls (changes equal/over 1.515-fold; p-values equal/lower 0.05). MS data revealed altered levels of structural membrane proteins (e.g. spectrins and ankyrin), components of the degradation machinery (proteasome subunits), and oxidative stress related proteins (e.g. catalase and peroxiredoxin-2) among others. Decreased catalase and peroxiredoxin-2 expression in RBC membrane of AAA patients compared to controls were further validated by Western blot, confirming the proteomic results. Conclusions and clinical relevance: RBCs membrane protein composition is altered in AAA patients, which could be involved in the pathological role of RBCs in aortic tissue and become potential targets to prevent AAA progression.

KW - Abdominal aortic aneurysm

KW - Red blood cells

UR - http://www.scopus.com/inward/record.url?scp=84906055554&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84906055554&partnerID=8YFLogxK

U2 - 10.1002/prca.201400035

DO - 10.1002/prca.201400035

M3 - Article

C2 - 24976601

AN - SCOPUS:84906055554

VL - 8

SP - 626

EP - 630

JO - Proteomics - Clinical Applications

JF - Proteomics - Clinical Applications

SN - 1862-8346

IS - 7-8

ER -