L-Triiodothyronine stimulates growth by means of an autocrine factor in a cultured growth hormone-producing cell line

M. J. Miller, E. C. Fels, L. E. Shapiro, Martin I. Surks

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

L-Triiodothyronine (T3) stimulates DNA synthesis and replication of cultured GC cells, a T3-responsive growth hormone (GH)-secreting cell line. To determine whether T3 stimulates secretion of an autocrine growth factor, we compared the growth-promoting activity of medium conditioned by T3-stimulated and T3-depleted cells to that of unconditioned medium. Addition of polyclonal rabbit anti-T3 serum to T3-containing media decreased cellular T3 content by 50-70%. In unconditioned medium, anti-T3 serum decreased T3-induced cell growth and GH production by 40-70%. In conditioned medium, anti-T3 serum also effected a 45-70% decrease in induction of GH secretion but did not attenuate the growth-promoting activity. Growth-promoting activity was not detected in medium conditioned by T3-depleted cells. Thus, conditioned medium from T3-containing GC cell cultures contains growth-promoting activity that is independent of T3. Further, the induction of GC cell growth by T3 may occur, at least in part, by induction of an autocrine growth factor.

Original languageEnglish (US)
Pages (from-to)1773-1781
Number of pages9
JournalJournal of Clinical Investigation
Volume79
Issue number6
StatePublished - 1987

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Triiodothyronine
Conditioned Culture Medium
Growth Hormone
Cell Line
Growth
Intercellular Signaling Peptides and Proteins
Serum
DNA Replication
Cultured Cells
Cell Culture Techniques
Rabbits
growth promoting activity

ASJC Scopus subject areas

  • Medicine(all)

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L-Triiodothyronine stimulates growth by means of an autocrine factor in a cultured growth hormone-producing cell line. / Miller, M. J.; Fels, E. C.; Shapiro, L. E.; Surks, Martin I.

In: Journal of Clinical Investigation, Vol. 79, No. 6, 1987, p. 1773-1781.

Research output: Contribution to journalArticle

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AU - Surks, Martin I.

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AB - L-Triiodothyronine (T3) stimulates DNA synthesis and replication of cultured GC cells, a T3-responsive growth hormone (GH)-secreting cell line. To determine whether T3 stimulates secretion of an autocrine growth factor, we compared the growth-promoting activity of medium conditioned by T3-stimulated and T3-depleted cells to that of unconditioned medium. Addition of polyclonal rabbit anti-T3 serum to T3-containing media decreased cellular T3 content by 50-70%. In unconditioned medium, anti-T3 serum decreased T3-induced cell growth and GH production by 40-70%. In conditioned medium, anti-T3 serum also effected a 45-70% decrease in induction of GH secretion but did not attenuate the growth-promoting activity. Growth-promoting activity was not detected in medium conditioned by T3-depleted cells. Thus, conditioned medium from T3-containing GC cell cultures contains growth-promoting activity that is independent of T3. Further, the induction of GC cell growth by T3 may occur, at least in part, by induction of an autocrine growth factor.

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