L-theanine protects the APP (Swedish mutation) transgenic SH-SY5Y cell against glutamate-induced excitotoxicity via inhibition of the NMDA receptor pathway

X. Di, Jingqi Yan, Y. Zhao, J. Zhang, Z. Shi, Y. Chang, B. Zhao

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

As a natural analogue of glutamate, l-theanine is the unique amino acid derivative in green tea. Although its underlining mechanisms are not yet clear, it has been suggested that l-theanine treatment may prove beneficial to patients with neurodegenerative diseases. In this study, we investigated the neuroprotective effect and its mechanism of l-theanine in an in vitro model of Alzheimer's disease by using the human APP (Swedish mutation) transgenic SH-SY5Y cell. Amyloid beta (Aβ) neurotoxicity was triggered by l-glutamate in this cell line. Additionally, l-theanine significantly attenuated l-glutamate-induced apoptosis at similar levels to those seen with the NMDA receptor inhibitor MK-801 in the stably expressing APP Swedish mutation SH-SY5Y cells which over-generated Aβ. Meanwhile, the activation of c-Jun N-terminal kinase and caspase-3 induced by l-glutamate was suppressed by l-theanine. We also found that cells treated with l-theanine showed decreased production of nitric oxide resulting from the down-regulated protein levels of inducible nitric oxide synthase (iNOS) and neuronal nitric oxide synthase (nNOS). These results indicate that the inhibition of the NMDA subtype of glutamate receptors and its related pathways is the crucial point of the neuroprotective effect of l-theanine in the cell model. Thus, our present study supports the notion that l-theanine may provide effective prophylaxis and treatment for Alzheimer's disease.

Original languageEnglish (US)
Pages (from-to)778-786
Number of pages9
JournalNeuroscience
Volume168
Issue number3
DOIs
StatePublished - Jul 1 2010
Externally publishedYes

Fingerprint

N-Methyl-D-Aspartate Receptors
Glutamic Acid
Mutation
Neuroprotective Agents
Mitogen-Activated Protein Kinase 10
Alzheimer Disease
Nitric Oxide Synthase Type I
Dizocilpine Maleate
Inhibition (Psychology)
theanine
Glutamate Receptors
Nitric Oxide Synthase Type II
Tea
N-Methylaspartate
Amyloid
Caspase 3
Neurodegenerative Diseases
Nitric Oxide
Apoptosis
Amino Acids

Keywords

  • Alzheimer's disease
  • Amyloid beta
  • Calcium
  • L-theanine
  • Neuroprotection
  • Nitric oxide

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

L-theanine protects the APP (Swedish mutation) transgenic SH-SY5Y cell against glutamate-induced excitotoxicity via inhibition of the NMDA receptor pathway. / Di, X.; Yan, Jingqi; Zhao, Y.; Zhang, J.; Shi, Z.; Chang, Y.; Zhao, B.

In: Neuroscience, Vol. 168, No. 3, 01.07.2010, p. 778-786.

Research output: Contribution to journalArticle

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