L-galactose metabolism in bacteroides vulgatus from the human gut microbiota

Merlin Eric Hobbs, Howard J. Williams, Brandan Hillerich, Steven C. Almo, Frank M. Raushel

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A previously unknown metabolic pathway for the utilization of l-galactose was discovered in a prevalent gut bacterium, Bacteroides vulgatus. The new pathway consists of three previously uncharacterized enzymes that were found to be responsible for the conversion of l-galactose to d-tagaturonate. Bvu0219 (l-galactose dehydrogenase) was determined to oxidize l-galactose to l-galactono-1,5-lactone with kcat and kcat/Km values of 21 s-1 and 2.0 × 105 M-1 s -1, respectively. The kinetic product of Bvu0219 is rapidly converted nonenzymatically to the thermodynamically more stable l-galactono-1,4-lactone. Bvu0220 (l-galactono-1,5-lactonase) hydrolyzes both the kinetic and thermodynamic products of Bvu0219 to l-galactonate. However, l-galactono-1,5-lactone is estimated to be hydrolyzed 300-fold faster than its thermodynamically more stable counterpart, l-galactono-1,4-lactone. In the final step of this pathway, Bvu0222 (l-galactonate dehydrogenase) oxidizes l-galactonate to d-tagaturonate with kcat and kcat/K m values of 0.6 s-1 and 1.7 × 104 M -1 s-1, respectively. In the reverse direction, d-tagaturonate is reduced to l-galactonate with values of kcat and kcat/Km of 90 s-1 and 1.6 × 10 5 M-1 s-1, respectively. d-Tagaturonate is subsequently converted to d-glyceraldehyde and pyruvate through enzymes encoded within the degradation pathway for d-glucuronate and d-galacturonate.

Original languageEnglish (US)
Pages (from-to)4661-4670
Number of pages10
JournalBiochemistry
Volume53
Issue number28
DOIs
StatePublished - Jul 22 2014

Fingerprint

Bacteroides
Galactose
Metabolism
galactose dehydrogenase
Lactones
Glyceraldehyde
Glucuronic Acid
Kinetics
Enzymes
Metabolic Networks and Pathways
Pyruvic Acid
Thermodynamics
Bacteria
Oxidoreductases
Degradation
Gastrointestinal Microbiome
galactono-1,4-lactone

ASJC Scopus subject areas

  • Biochemistry

Cite this

Hobbs, M. E., Williams, H. J., Hillerich, B., Almo, S. C., & Raushel, F. M. (2014). L-galactose metabolism in bacteroides vulgatus from the human gut microbiota. Biochemistry, 53(28), 4661-4670. https://doi.org/10.1021/bi500656m

L-galactose metabolism in bacteroides vulgatus from the human gut microbiota. / Hobbs, Merlin Eric; Williams, Howard J.; Hillerich, Brandan; Almo, Steven C.; Raushel, Frank M.

In: Biochemistry, Vol. 53, No. 28, 22.07.2014, p. 4661-4670.

Research output: Contribution to journalArticle

Hobbs, ME, Williams, HJ, Hillerich, B, Almo, SC & Raushel, FM 2014, 'L-galactose metabolism in bacteroides vulgatus from the human gut microbiota', Biochemistry, vol. 53, no. 28, pp. 4661-4670. https://doi.org/10.1021/bi500656m
Hobbs, Merlin Eric ; Williams, Howard J. ; Hillerich, Brandan ; Almo, Steven C. ; Raushel, Frank M. / L-galactose metabolism in bacteroides vulgatus from the human gut microbiota. In: Biochemistry. 2014 ; Vol. 53, No. 28. pp. 4661-4670.
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