KpnI families of long, interspersed repetitive DNAs associated with the human β-globin gene cluster

Bridget Shafit-Zagardo, Fred L. Brown, Joseph J. Maio, Jesse W. Adams

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35 Scopus citations

Abstract

KpnI families of long, interspersed repetitive DNAs are ubiquitous repetitive elements that occur in tens of thousands of copies in primate genomes. KpnI 1.2, 1.5 and two different KpnI 1.8-kb families were found within and flanking a 6.4-kb repeat beginning at 3 kb, 3' from the human β-globin gene. Thus, six different types of KpnI families have now been identified, and four of these are found next to each other in a specific 6.4-kb repeat. Clones of the distinct KpnI families were hybridized to clones of the 6.4-kb repeat and adjacent sequences encompassed within some 17.6 kb of DNA lying 3' to the β-globin gene cluster. The four KpnI families appear to make up the entire length of the 6.4-kb repeat. The linear order of the various cloned KpnI sequences in the repeat is 5'-pBK(1.8)26-pBK.(1.5)54-pBK(1.2)11-pBK(1.8)11-3'. KpnI 1.2-kb sequences were also detected downstream from the 6.4-kb repeat. As in the case of the KpnI 1.2 and 1.5-kb families, the two KpnI 1.8-kb sequence families described here each hybridized with about 15% of all plaques in two independently generated human genome libraries.

Original languageEnglish (US)
Pages (from-to)397-407
Number of pages11
JournalGene
Volume20
Issue number3
DOIs
Publication statusPublished - Dec 1982
Externally publishedYes

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Keywords

  • Human hemoglobin genes
  • gene regulation
  • recombinant DNA

ASJC Scopus subject areas

  • Genetics

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