Kinetics of conformational transitions of demetalized Concanavalin A

C. Fred Brewer, Rodney D. Brown, Seymour H. Koenig

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Demetalized Concanavalin A exists in two conformational states, known as locked (PL) and unlocked (P) [Brown et al., Biochemistry 16, 3883 (1977)]. The equilibrium ratio [PL] [P] is 0.14±0.01 at 25 °C, pH 6.4 [Brown et al., Biochemistry 21, 465 (1982)]. We now report values of the rate constants for the P ái PL equilibrium; k1 = (33±4 h)-1 and k-1 = (4.6±0.6 h)-1 for the P → PL and PL → P transitions, respectively, at 25 °C, pH 6.4. The experiments utilize the fact that saccharide binds to PL [Koenig et al., Biochemistry 17, 4251 (1978)], producing a time-dependent increase in the total concentration of locked forms at equilibrium, and use a new technique for measuring this concentration.

Original languageEnglish (US)
Pages (from-to)595-601
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume112
Issue number2
DOIs
StatePublished - Apr 29 1983

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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