Kinetic mechanism and nucleotide specificity of NADH peroxidase

Vincent S. Stoll, John S. Blanchard

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

NADH peroxidase is a flavoprotein isolated from Streptococcus faecalis which catalyzes the pyridine nucleotide-dependent reduction of hydrogen peroxide to water. Initial velocity, product, and dead-end inhibition studies have been performed at pH 7.5 and support a ping-pong kinetic mechanism. In the absence of hydrogen peroxide, both transhydrogenation between NADH and thioNAD, and isotope exchange between [14C]NADH and NAD, have been demonstrated, although in both these experiments, the maximal velocity of nucleotide exchange was less than 1.5% the maximal velocity of the peroxidatic reaction. We propose that NADH binds tightly to both oxidized and twoelectron reduced enzyme. NADH oxidation proceeds stereospecifically with the transfer of the 4S hydrogen to enzyme, and then, via exchange, to water. No primary tritium kinetic isotope effect was observed, and no statistically significant primary deuterium kinetic isotope effects on V K were determined, although primary deuterium kinetic isotope effects on V were observed in the presence and absence of sodium acetate. NADH peroxidase thus shares with other flavoprotein reductases striking kinetic, spectroscopic, and stereochemical similarities. On this basis, we propose a chemical mechanism for the peroxide cleaving reaction catalyzed by NADH peroxidase which involves the obligate formation of a flavinperoxide, and peroxo bond cleavage by nucleophilic attack by enzymatic dithiols.

Original languageEnglish (US)
Pages (from-to)752-762
Number of pages11
JournalArchives of Biochemistry and Biophysics
Volume260
Issue number2
DOIs
StatePublished - Feb 1 1988

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NAD+ peroxidase
NAD
Nucleotides
Isotopes
Kinetics
Flavoproteins
Deuterium
Hydrogen Peroxide
Sodium Acetate
Tritium
Water
Enterococcus faecalis
Peroxides
Enzymes
Hydrogen
Oxidoreductases
Oxidation

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Kinetic mechanism and nucleotide specificity of NADH peroxidase. / Stoll, Vincent S.; Blanchard, John S.

In: Archives of Biochemistry and Biophysics, Vol. 260, No. 2, 01.02.1988, p. 752-762.

Research output: Contribution to journalArticle

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