Abstract
Kinetic studies were conducted on the contractile response elicited by phenylephrine (PE) activation of the α1-adrenergic receptor subtype in vascular smooth muscle isolated from the corpus cavernosum of impotent men. PE-induced contractions were separated into distinct phasic and tonic components, and the tonic portion was analyzed using a first-order rate equation to determine the maximal rate constant for onset of contraction (k(obs max)) and the maximum amplitude of the steady-state contractile response (R(eq max)). The k(obs max) value in tissues from insulin-dependent diabetic patients was significantly greater than that in tissues from either noninsulin-dependent diabetics or nondiabetics. Additionally, the mean k(obs max) value in older patients (60-70 yr) was significantly greater than the mean k(obs max) value in younger patients (32-59 yr). Significant diabetes- related, but not age-related, alterations were also found in R(eq max). The observed changes in contractility resulted in dramatic age- and pathology- dependent alterations in the initial rate and/or magnitude of PE-induced response generation. These kinetic studies extend our previous observations at steady state and provide further evidence for heightened corporal tissue tone in the etiology of erectile dysfunction.
Original language | English (US) |
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Pages (from-to) | H15-H19 |
Journal | American Journal of Physiology - Heart and Circulatory Physiology |
Volume | 263 |
Issue number | 1 32-1 |
State | Published - 1992 |
Keywords
- erectile dysfunction
- kinetics
- phenylephrine
- vascular smooth muscle
ASJC Scopus subject areas
- Physiology
- Cardiology and Cardiovascular Medicine
- Physiology (medical)