Junctional communication is induced in migrating capillary endothelial cells

M. S. Pepper, David C. Spray, M. Chanson, R. Montesano, L. Orci, P. Meda

Research output: Contribution to journalArticle

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Abstract

Using an in vitro model in which a confluent monolayer of capillary enothelial cells is mechanically wounded, gap junction-mediated intercellular communication has been studied by loading the cells with the fluorescent dye, Lucifer Yellow. Approximately 40-50% of the cells in a nonwounded confluent monolayer were coupled in groups of four to five cells (basal level). Basal levels of communication were also observed in sparse and preconfluent cultures, but were reduced in postconfluent monolayers. 30 min after wounding, coupling was markedly reduced between cells lining the wound. Communication at the wound was partially reestablished by 2 h, exceeded basal levels after 6 h and reached a maximum after 24 h, at which stage ~90% of the cells were coupled in groups of six to seven cells. When the wound had closed (after 8 d), the increase in communication was no longer observed. Induction of wound-associated communication was unaffected by exposure of the cells to the DNA synthesis inhibitor mitomycin C, but was prevented by the protein synthesis inhibitor, cycloheximide. The induction of wound-associated communication was also inhibited when migration was prevented by placing the cells immediately after wounding at 22°C or after exposure to cytochalasin D, suggesting that the increase in communication is dependent on cells migrating into the wound area. In contrast, migration was not prevented when coupling was blocked by exposure of the cells to retinoic acid, although this agent did disrupt the characteristic sheet-like pattern of migration typically seen during endothelial repair. These results suggest that junctional communication may play an important role in wound repair, possibly by coordinating capillary endothelial cell migration.

Original languageEnglish (US)
Pages (from-to)3027-3038
Number of pages12
JournalJournal of Cell Biology
Volume109
Issue number6 I
DOIs
StatePublished - 1989
Externally publishedYes

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Endothelial Cells
Wounds and Injuries
Nucleic Acid Synthesis Inhibitors
Cytochalasin D
Protein Synthesis Inhibitors
Gap Junctions
Mitomycin
Cycloheximide
Tretinoin
Fluorescent Dyes
Cell Movement

ASJC Scopus subject areas

  • Cell Biology

Cite this

Junctional communication is induced in migrating capillary endothelial cells. / Pepper, M. S.; Spray, David C.; Chanson, M.; Montesano, R.; Orci, L.; Meda, P.

In: Journal of Cell Biology, Vol. 109, No. 6 I, 1989, p. 3027-3038.

Research output: Contribution to journalArticle

Pepper, MS, Spray, DC, Chanson, M, Montesano, R, Orci, L & Meda, P 1989, 'Junctional communication is induced in migrating capillary endothelial cells', Journal of Cell Biology, vol. 109, no. 6 I, pp. 3027-3038. https://doi.org/10.1083/jcb.109.6.3027
Pepper, M. S. ; Spray, David C. ; Chanson, M. ; Montesano, R. ; Orci, L. ; Meda, P. / Junctional communication is induced in migrating capillary endothelial cells. In: Journal of Cell Biology. 1989 ; Vol. 109, No. 6 I. pp. 3027-3038.
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