Joint composite-rotation adiabatic-sweep isotope filtration

Elizabeth R. Valentine; Fabien Ferrage; Francesca Massi; David Cowburn; Arthur G. Palmer

doi:10.1007/s10858-006-9131-9

Joint composite-rotation adiabatic-sweep isotope filtration

Elizabeth R. Valentine, Fabien Ferrage, Francesca Massi, David Cowburn, Arthur G. Palmer

Biochemistry

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Joint composite-rotation adiabatic-sweep isotope filters are derived by combining the composite-rotation [Stuart AC et al. (1999) J Am Chem Soc 121: 5346-5347] and adiabatic-sweep [Zwahlen C et al. (1997) J Am Chem Soc 119:6711-6721; Kup̂e E, Freeman R (1997) J Magn Reson 127:36-48] approaches. The joint isotope filters have improved broadband filtration performance, even for extreme values of the one-bond ¹H-¹³C scalar coupling constants in proteins and RNA molecules. An average Hamiltonian analysis is used to describe evolution of the heteronuclear scalar coupling interaction during the adiabatic sweeps within the isotope filter sequences. The new isotope filter elements permit improved selective detection of NMR resonance signals originating from ¹H spins attached to an unlabeled natural abundance component of a complex in which the other components are labeled with ¹³C and ¹⁵N isotopes.

Original language	English (US)
Pages (from-to)	11-22
Number of pages	12
Journal	Journal of Biomolecular NMR
Volume	38
Issue number	1
DOIs	https://doi.org/10.1007/s10858-006-9131-9
State	Published - May 2007

Keywords

Composite pulse
J-filter
NOESY
Structure determination
Vts SAM domain

ASJC Scopus subject areas

Biochemistry
Spectroscopy

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10.1007/s10858-006-9131-9

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title = "Joint composite-rotation adiabatic-sweep isotope filtration",

abstract = "Joint composite-rotation adiabatic-sweep isotope filters are derived by combining the composite-rotation [Stuart AC et al. (1999) J Am Chem Soc 121: 5346-5347] and adiabatic-sweep [Zwahlen C et al. (1997) J Am Chem Soc 119:6711-6721; Ku{\^p}e E, Freeman R (1997) J Magn Reson 127:36-48] approaches. The joint isotope filters have improved broadband filtration performance, even for extreme values of the one-bond 1H-13C scalar coupling constants in proteins and RNA molecules. An average Hamiltonian analysis is used to describe evolution of the heteronuclear scalar coupling interaction during the adiabatic sweeps within the isotope filter sequences. The new isotope filter elements permit improved selective detection of NMR resonance signals originating from 1H spins attached to an unlabeled natural abundance component of a complex in which the other components are labeled with 13C and 15N isotopes.",

keywords = "Composite pulse, J-filter, NOESY, Structure determination, Vts SAM domain",

author = "Valentine, {Elizabeth R.} and Fabien Ferrage and Francesca Massi and David Cowburn and Palmer, {Arthur G.}",

note = "Funding Information: Acknowledgements This work was supported by NIH grants GM50291 (A.G. P.) and GM47021 (D. C.). E.R.V. acknowledges support from a National Science Foundation Graduate Research Fellowship. We thank Joel A. Butterwick (Columbia University), Thomas A. Edwards (Mt. Sinai School of Medicine), and Aneel K. Aggarwal (Mt. Sinai School of Medicine) for the Vts1 SAM domain/TCE 13mer RNA sample. Helpful discussions with Mark Rance (Univ. Cincinnati) are acknowledged gratefully.",

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doi = "10.1007/s10858-006-9131-9",

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AU - Ferrage, Fabien

AU - Massi, Francesca

AU - Cowburn, David

AU - Palmer, Arthur G.

N1 - Funding Information: Acknowledgements This work was supported by NIH grants GM50291 (A.G. P.) and GM47021 (D. C.). E.R.V. acknowledges support from a National Science Foundation Graduate Research Fellowship. We thank Joel A. Butterwick (Columbia University), Thomas A. Edwards (Mt. Sinai School of Medicine), and Aneel K. Aggarwal (Mt. Sinai School of Medicine) for the Vts1 SAM domain/TCE 13mer RNA sample. Helpful discussions with Mark Rance (Univ. Cincinnati) are acknowledged gratefully.

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N2 - Joint composite-rotation adiabatic-sweep isotope filters are derived by combining the composite-rotation [Stuart AC et al. (1999) J Am Chem Soc 121: 5346-5347] and adiabatic-sweep [Zwahlen C et al. (1997) J Am Chem Soc 119:6711-6721; Kup̂e E, Freeman R (1997) J Magn Reson 127:36-48] approaches. The joint isotope filters have improved broadband filtration performance, even for extreme values of the one-bond 1H-13C scalar coupling constants in proteins and RNA molecules. An average Hamiltonian analysis is used to describe evolution of the heteronuclear scalar coupling interaction during the adiabatic sweeps within the isotope filter sequences. The new isotope filter elements permit improved selective detection of NMR resonance signals originating from 1H spins attached to an unlabeled natural abundance component of a complex in which the other components are labeled with 13C and 15N isotopes.

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Joint composite-rotation adiabatic-sweep isotope filtration

Abstract

Keywords

ASJC Scopus subject areas

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