Joint chromatographic purification of bovine serum ceruloplasmin and amineoxidase

X. T. Wang, M. J. Dumoulin, O. Befani, B. Mondovi, M. A. Mateescu

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27 Scopus citations

Abstract

A purification procedure leading to a joint separation of two serum copper-enzymes: ceruloplasmin (EC 1.16.3.1) and amineoxidase (EC 1.4.3.6), is described. Both enzymes are obtained in electrophoretically homogeneous form and their specific activities are higher than those obtained by previously described purification techniques. Two common steps: precipitation of bovine plasma proteins with ammonium sulphate (at 35 % and 55 % saturation) followed by column chromatography on AE-Agarose (obtained by treatment of agarose beads with l-chloro-2-ethylamine), lead to an electrophoretically homogeneous ceruloplasmin. At the same time, the ceruloplasmin-free protein preparation eluted in a first peak, following further Q-Sepharose and Con A-Sepharose chromatography, leads to purified bovine serum amine oxidase (BSAO) with an improved yield. The emphasis was given to a mutual improving effect as a consequence of the integration of the two enzymes purification procedures.

Original languageEnglish (US)
Pages (from-to)237-250
Number of pages14
JournalPreparative Biochemistry
Volume24
Issue number3-4
DOIs
StatePublished - Nov 1 1994

ASJC Scopus subject areas

  • Biochemistry
  • Genetics

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